Neuroinductive properties of mGDNF depend on the producer, E. Coli or human cells.

The glial cell line-derived neurotrophic factor (GDNF) is involved in the survival of dopaminergic neurons. Besides, GDNF can also induce axonal growth and creation of new functional synapses. GDNF potential is promising for translation to treat diseases associated with neuronal death: neurodegenera...

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Autores principales:	Dzhirgala V Shamadykova, Dmitry Y Panteleev, Nadezhda N Kust, Ekaterina A Savchenko, Ekaterina Y Rybalkina, Alexander V Revishchin, Galina V Pavlova
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/767fcb299b8c4227b76c2a11ba335bee
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spelling	oai:doaj.org-article:767fcb299b8c4227b76c2a11ba335bee2021-12-02T20:17:03ZNeuroinductive properties of mGDNF depend on the producer, E. Coli or human cells.1932-620310.1371/journal.pone.0258289https://doaj.org/article/767fcb299b8c4227b76c2a11ba335bee2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0258289https://doaj.org/toc/1932-6203The glial cell line-derived neurotrophic factor (GDNF) is involved in the survival of dopaminergic neurons. Besides, GDNF can also induce axonal growth and creation of new functional synapses. GDNF potential is promising for translation to treat diseases associated with neuronal death: neurodegenerative disorders, ischemic stroke, and cerebral or spinal cord damages. Unproductive clinical trials of GDNF for Parkinson's disease treatment have induced to study this failure. A reason could be due to irrelevant producer cells that cannot perform the required post-translational modifications. The biological activity of recombinant mGDNF produced by E. coli have been compared with mGDNF produced by human cells HEK293. mGDNF variants were tested with PC12 cells, rat embryonic spinal ganglion cells, and SH-SY5Y human neuroblastoma cells in vitro as well as with a mouse model of the Parkinson's disease in vivo. Both in vitro and in vivo the best neuro-inductive ability belongs to mGDNF produced by HEK293 cells. Keywords: GDNF, neural differentiation, bacterial and mammalian expression systems, cell cultures, model of Parkinson's disease.Dzhirgala V ShamadykovaDmitry Y PanteleevNadezhda N KustEkaterina A SavchenkoEkaterina Y RybalkinaAlexander V RevishchinGalina V PavlovaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 10, p e0258289 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dzhirgala V Shamadykova Dmitry Y Panteleev Nadezhda N Kust Ekaterina A Savchenko Ekaterina Y Rybalkina Alexander V Revishchin Galina V Pavlova Neuroinductive properties of mGDNF depend on the producer, E. Coli or human cells.
description	The glial cell line-derived neurotrophic factor (GDNF) is involved in the survival of dopaminergic neurons. Besides, GDNF can also induce axonal growth and creation of new functional synapses. GDNF potential is promising for translation to treat diseases associated with neuronal death: neurodegenerative disorders, ischemic stroke, and cerebral or spinal cord damages. Unproductive clinical trials of GDNF for Parkinson's disease treatment have induced to study this failure. A reason could be due to irrelevant producer cells that cannot perform the required post-translational modifications. The biological activity of recombinant mGDNF produced by E. coli have been compared with mGDNF produced by human cells HEK293. mGDNF variants were tested with PC12 cells, rat embryonic spinal ganglion cells, and SH-SY5Y human neuroblastoma cells in vitro as well as with a mouse model of the Parkinson's disease in vivo. Both in vitro and in vivo the best neuro-inductive ability belongs to mGDNF produced by HEK293 cells. Keywords: GDNF, neural differentiation, bacterial and mammalian expression systems, cell cultures, model of Parkinson's disease.
format	article
author	Dzhirgala V Shamadykova Dmitry Y Panteleev Nadezhda N Kust Ekaterina A Savchenko Ekaterina Y Rybalkina Alexander V Revishchin Galina V Pavlova
author_facet	Dzhirgala V Shamadykova Dmitry Y Panteleev Nadezhda N Kust Ekaterina A Savchenko Ekaterina Y Rybalkina Alexander V Revishchin Galina V Pavlova
author_sort	Dzhirgala V Shamadykova
title	Neuroinductive properties of mGDNF depend on the producer, E. Coli or human cells.
title_short	Neuroinductive properties of mGDNF depend on the producer, E. Coli or human cells.
title_full	Neuroinductive properties of mGDNF depend on the producer, E. Coli or human cells.
title_fullStr	Neuroinductive properties of mGDNF depend on the producer, E. Coli or human cells.
title_full_unstemmed	Neuroinductive properties of mGDNF depend on the producer, E. Coli or human cells.
title_sort	neuroinductive properties of mgdnf depend on the producer, e. coli or human cells.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/767fcb299b8c4227b76c2a11ba335bee
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Neuroinductive properties of mGDNF depend on the producer, E. Coli or human cells.

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