Small molecule inhibition of ATM kinase increases CRISPR-Cas9 1-bp insertion frequency

The mutational outcome of CRISPR-Cas9 editing can be both predictable and targeted. Here the authors show that ATM inhibitor KU-60019 increases 1 bp insertions at the targeted locus.

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Autores principales:	Heysol C. Bermudez-Cabrera, Sannie Culbertson, Sammy Barkal, Benjamin Holmes, Max W. Shen, Sophia Zhang, David K. Gifford, Richard I. Sherwood
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/77675116590e4b8aa7e1d10c6a4309b0
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spelling	oai:doaj.org-article:77675116590e4b8aa7e1d10c6a4309b02021-12-02T15:09:09ZSmall molecule inhibition of ATM kinase increases CRISPR-Cas9 1-bp insertion frequency10.1038/s41467-021-25415-82041-1723https://doaj.org/article/77675116590e4b8aa7e1d10c6a4309b02021-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-25415-8https://doaj.org/toc/2041-1723The mutational outcome of CRISPR-Cas9 editing can be both predictable and targeted. Here the authors show that ATM inhibitor KU-60019 increases 1 bp insertions at the targeted locus.Heysol C. Bermudez-CabreraSannie CulbertsonSammy BarkalBenjamin HolmesMax W. ShenSophia ZhangDavid K. GiffordRichard I. SherwoodNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Heysol C. Bermudez-Cabrera Sannie Culbertson Sammy Barkal Benjamin Holmes Max W. Shen Sophia Zhang David K. Gifford Richard I. Sherwood Small molecule inhibition of ATM kinase increases CRISPR-Cas9 1-bp insertion frequency
description	The mutational outcome of CRISPR-Cas9 editing can be both predictable and targeted. Here the authors show that ATM inhibitor KU-60019 increases 1 bp insertions at the targeted locus.
format	article
author	Heysol C. Bermudez-Cabrera Sannie Culbertson Sammy Barkal Benjamin Holmes Max W. Shen Sophia Zhang David K. Gifford Richard I. Sherwood
author_facet	Heysol C. Bermudez-Cabrera Sannie Culbertson Sammy Barkal Benjamin Holmes Max W. Shen Sophia Zhang David K. Gifford Richard I. Sherwood
author_sort	Heysol C. Bermudez-Cabrera
title	Small molecule inhibition of ATM kinase increases CRISPR-Cas9 1-bp insertion frequency
title_short	Small molecule inhibition of ATM kinase increases CRISPR-Cas9 1-bp insertion frequency
title_full	Small molecule inhibition of ATM kinase increases CRISPR-Cas9 1-bp insertion frequency
title_fullStr	Small molecule inhibition of ATM kinase increases CRISPR-Cas9 1-bp insertion frequency
title_full_unstemmed	Small molecule inhibition of ATM kinase increases CRISPR-Cas9 1-bp insertion frequency
title_sort	small molecule inhibition of atm kinase increases crispr-cas9 1-bp insertion frequency
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/77675116590e4b8aa7e1d10c6a4309b0
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Small molecule inhibition of ATM kinase increases CRISPR-Cas9 1-bp insertion frequency

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