PhotoGate microscopy to track single molecules in crowded environments
Tracking single molecules in living cells is difficult or impractical owing to their high density. Here the authors introduce a technique of repeatedly photobleaching fluorophores at the boundary of a region of interest, thereby controlling the number of fluorescent particles for observation.
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Auteurs principaux: | , , , , , , |
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Format: | article |
Langue: | EN |
Publié: |
Nature Portfolio
2017
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Sujets: | |
Accès en ligne: | https://doaj.org/article/77efbc9571114b969331b9cd398322cf |
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