PhotoGate microscopy to track single molecules in crowded environments

Tracking single molecules in living cells is difficult or impractical owing to their high density. Here the authors introduce a technique of repeatedly photobleaching fluorophores at the boundary of a region of interest, thereby controlling the number of fluorescent particles for observation.

Enregistré dans:
Détails bibliographiques
Auteurs principaux: Vladislav Belyy, Sheng-Min Shih, Jigar Bandaria, Yongjian Huang, Rosalie E. Lawrence, Roberto Zoncu, Ahmet Yildiz
Format: article
Langue:EN
Publié: Nature Portfolio 2017
Sujets:
Q
Accès en ligne:https://doaj.org/article/77efbc9571114b969331b9cd398322cf
Tags: Ajouter un tag
Pas de tags, Soyez le premier à ajouter un tag!