PhotoGate microscopy to track single molecules in crowded environments

Tracking single molecules in living cells is difficult or impractical owing to their high density. Here the authors introduce a technique of repeatedly photobleaching fluorophores at the boundary of a region of interest, thereby controlling the number of fluorescent particles for observation.

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Detalles Bibliográficos
Autores principales: Vladislav Belyy, Sheng-Min Shih, Jigar Bandaria, Yongjian Huang, Rosalie E. Lawrence, Roberto Zoncu, Ahmet Yildiz
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/77efbc9571114b969331b9cd398322cf
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Descripción
Sumario:Tracking single molecules in living cells is difficult or impractical owing to their high density. Here the authors introduce a technique of repeatedly photobleaching fluorophores at the boundary of a region of interest, thereby controlling the number of fluorescent particles for observation.