PhotoGate microscopy to track single molecules in crowded environments

Tracking single molecules in living cells is difficult or impractical owing to their high density. Here the authors introduce a technique of repeatedly photobleaching fluorophores at the boundary of a region of interest, thereby controlling the number of fluorescent particles for observation.

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Autores principales:	Vladislav Belyy, Sheng-Min Shih, Jigar Bandaria, Yongjian Huang, Rosalie E. Lawrence, Roberto Zoncu, Ahmet Yildiz
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/77efbc9571114b969331b9cd398322cf
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spelling	oai:doaj.org-article:77efbc9571114b969331b9cd398322cf2021-12-02T14:41:10ZPhotoGate microscopy to track single molecules in crowded environments10.1038/ncomms139782041-1723https://doaj.org/article/77efbc9571114b969331b9cd398322cf2017-01-01T00:00:00Zhttps://doi.org/10.1038/ncomms13978https://doaj.org/toc/2041-1723Tracking single molecules in living cells is difficult or impractical owing to their high density. Here the authors introduce a technique of repeatedly photobleaching fluorophores at the boundary of a region of interest, thereby controlling the number of fluorescent particles for observation.Vladislav BelyySheng-Min ShihJigar BandariaYongjian HuangRosalie E. LawrenceRoberto ZoncuAhmet YildizNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Vladislav Belyy Sheng-Min Shih Jigar Bandaria Yongjian Huang Rosalie E. Lawrence Roberto Zoncu Ahmet Yildiz PhotoGate microscopy to track single molecules in crowded environments
description	Tracking single molecules in living cells is difficult or impractical owing to their high density. Here the authors introduce a technique of repeatedly photobleaching fluorophores at the boundary of a region of interest, thereby controlling the number of fluorescent particles for observation.
format	article
author	Vladislav Belyy Sheng-Min Shih Jigar Bandaria Yongjian Huang Rosalie E. Lawrence Roberto Zoncu Ahmet Yildiz
author_facet	Vladislav Belyy Sheng-Min Shih Jigar Bandaria Yongjian Huang Rosalie E. Lawrence Roberto Zoncu Ahmet Yildiz
author_sort	Vladislav Belyy
title	PhotoGate microscopy to track single molecules in crowded environments
title_short	PhotoGate microscopy to track single molecules in crowded environments
title_full	PhotoGate microscopy to track single molecules in crowded environments
title_fullStr	PhotoGate microscopy to track single molecules in crowded environments
title_full_unstemmed	PhotoGate microscopy to track single molecules in crowded environments
title_sort	photogate microscopy to track single molecules in crowded environments
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/77efbc9571114b969331b9cd398322cf
work_keys_str_mv	AT vladislavbelyy photogatemicroscopytotracksinglemoleculesincrowdedenvironments AT shengminshih photogatemicroscopytotracksinglemoleculesincrowdedenvironments AT jigarbandaria photogatemicroscopytotracksinglemoleculesincrowdedenvironments AT yongjianhuang photogatemicroscopytotracksinglemoleculesincrowdedenvironments AT rosalieelawrence photogatemicroscopytotracksinglemoleculesincrowdedenvironments AT robertozoncu photogatemicroscopytotracksinglemoleculesincrowdedenvironments AT ahmetyildiz photogatemicroscopytotracksinglemoleculesincrowdedenvironments
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PhotoGate microscopy to track single molecules in crowded environments

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