Designing PCR Primers Painlessly

Students design primers using web-based tools along with a printed sequence of a prokaryotic gene showing both DNA strands and translation of the coding strand into amino acids. The melting temperature of each primer is determined and the pair closest in temperature can be used to amplify and clone...

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Autores principales: Morgan Feeney, Kevin Murphy, Jane Lopilato
Formato: article
Lenguaje:EN
Publicado: American Society for Microbiology 2014
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Acceso en línea:https://doaj.org/article/799f283e04d544c09c4f4f8dec07db15
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