Designing PCR Primers Painlessly

Students design primers using web-based tools along with a printed sequence of a prokaryotic gene showing both DNA strands and translation of the coding strand into amino acids. The melting temperature of each primer is determined and the pair closest in temperature can be used to amplify and clone...

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Autores principales:	Morgan Feeney, Kevin Murphy, Jane Lopilato
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2014
Materias:	Special aspects of education LC8-6691 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/799f283e04d544c09c4f4f8dec07db15
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spelling	oai:doaj.org-article:799f283e04d544c09c4f4f8dec07db152021-11-15T15:03:36ZDesigning PCR Primers Painlessly10.1128/jmbe.v15i1.6341935-78851935-7877https://doaj.org/article/799f283e04d544c09c4f4f8dec07db152014-05-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/jmbe.v15i1.634https://doaj.org/toc/1935-7877https://doaj.org/toc/1935-7885Students design primers using web-based tools along with a printed sequence of a prokaryotic gene showing both DNA strands and translation of the coding strand into amino acids. The melting temperature of each primer is determined and the pair closest in temperature can be used to amplify and clone a gene into a vector for a one-semester laboratory exercise. By working through this primer design exercise, students see first hand what is involved in the process of amplifying DNA.Morgan FeeneyKevin MurphyJane LopilatoAmerican Society for MicrobiologyarticleSpecial aspects of educationLC8-6691Biology (General)QH301-705.5ENJournal of Microbiology & Biology Education, Vol 15, Iss 1, Pp 28-29 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Special aspects of education LC8-6691 Biology (General) QH301-705.5
spellingShingle	Special aspects of education LC8-6691 Biology (General) QH301-705.5 Morgan Feeney Kevin Murphy Jane Lopilato Designing PCR Primers Painlessly
description	Students design primers using web-based tools along with a printed sequence of a prokaryotic gene showing both DNA strands and translation of the coding strand into amino acids. The melting temperature of each primer is determined and the pair closest in temperature can be used to amplify and clone a gene into a vector for a one-semester laboratory exercise. By working through this primer design exercise, students see first hand what is involved in the process of amplifying DNA.
format	article
author	Morgan Feeney Kevin Murphy Jane Lopilato
author_facet	Morgan Feeney Kevin Murphy Jane Lopilato
author_sort	Morgan Feeney
title	Designing PCR Primers Painlessly
title_short	Designing PCR Primers Painlessly
title_full	Designing PCR Primers Painlessly
title_fullStr	Designing PCR Primers Painlessly
title_full_unstemmed	Designing PCR Primers Painlessly
title_sort	designing pcr primers painlessly
publisher	American Society for Microbiology
publishDate	2014
url	https://doaj.org/article/799f283e04d544c09c4f4f8dec07db15
work_keys_str_mv	AT morganfeeney designingpcrprimerspainlessly AT kevinmurphy designingpcrprimerspainlessly AT janelopilato designingpcrprimerspainlessly
_version_	1718428321248706560

Designing PCR Primers Painlessly

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