RNA-aptamers-in-droplets (RAPID) high-throughput screening for secretory phenotypes

Screening libraries of genetically engineered microbes for secreted products is limited by the available assay throughput. Here the authors combine aptamer-based fluorescent detection with droplet microfluidics to achieve high throughput screening of yeast strains engineered for enhanced tyrosine or...

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Auteurs principaux: Joseph Abatemarco, Maen F. Sarhan, James M. Wagner, Jyun-Liang Lin, Leqian Liu, Wafa Hassouneh, Shuo-Fu Yuan, Hal S. Alper, Adam R. Abate
Format: article
Langue:EN
Publié: Nature Portfolio 2017
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Accès en ligne:https://doaj.org/article/7a394be6725a4ab49a545fcea90ff67c
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