RNA-aptamers-in-droplets (RAPID) high-throughput screening for secretory phenotypes

Screening libraries of genetically engineered microbes for secreted products is limited by the available assay throughput. Here the authors combine aptamer-based fluorescent detection with droplet microfluidics to achieve high throughput screening of yeast strains engineered for enhanced tyrosine or...

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Autores principales:	Joseph Abatemarco, Maen F. Sarhan, James M. Wagner, Jyun-Liang Lin, Leqian Liu, Wafa Hassouneh, Shuo-Fu Yuan, Hal S. Alper, Adam R. Abate
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/7a394be6725a4ab49a545fcea90ff67c
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spelling	oai:doaj.org-article:7a394be6725a4ab49a545fcea90ff67c2021-12-02T14:16:55ZRNA-aptamers-in-droplets (RAPID) high-throughput screening for secretory phenotypes10.1038/s41467-017-00425-72041-1723https://doaj.org/article/7a394be6725a4ab49a545fcea90ff67c2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-017-00425-7https://doaj.org/toc/2041-1723Screening libraries of genetically engineered microbes for secreted products is limited by the available assay throughput. Here the authors combine aptamer-based fluorescent detection with droplet microfluidics to achieve high throughput screening of yeast strains engineered for enhanced tyrosine or streptavidin production.Joseph AbatemarcoMaen F. SarhanJames M. WagnerJyun-Liang LinLeqian LiuWafa HassounehShuo-Fu YuanHal S. AlperAdam R. AbateNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Joseph Abatemarco Maen F. Sarhan James M. Wagner Jyun-Liang Lin Leqian Liu Wafa Hassouneh Shuo-Fu Yuan Hal S. Alper Adam R. Abate RNA-aptamers-in-droplets (RAPID) high-throughput screening for secretory phenotypes
description	Screening libraries of genetically engineered microbes for secreted products is limited by the available assay throughput. Here the authors combine aptamer-based fluorescent detection with droplet microfluidics to achieve high throughput screening of yeast strains engineered for enhanced tyrosine or streptavidin production.
format	article
author	Joseph Abatemarco Maen F. Sarhan James M. Wagner Jyun-Liang Lin Leqian Liu Wafa Hassouneh Shuo-Fu Yuan Hal S. Alper Adam R. Abate
author_facet	Joseph Abatemarco Maen F. Sarhan James M. Wagner Jyun-Liang Lin Leqian Liu Wafa Hassouneh Shuo-Fu Yuan Hal S. Alper Adam R. Abate
author_sort	Joseph Abatemarco
title	RNA-aptamers-in-droplets (RAPID) high-throughput screening for secretory phenotypes
title_short	RNA-aptamers-in-droplets (RAPID) high-throughput screening for secretory phenotypes
title_full	RNA-aptamers-in-droplets (RAPID) high-throughput screening for secretory phenotypes
title_fullStr	RNA-aptamers-in-droplets (RAPID) high-throughput screening for secretory phenotypes
title_full_unstemmed	RNA-aptamers-in-droplets (RAPID) high-throughput screening for secretory phenotypes
title_sort	rna-aptamers-in-droplets (rapid) high-throughput screening for secretory phenotypes
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/7a394be6725a4ab49a545fcea90ff67c
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RNA-aptamers-in-droplets (RAPID) high-throughput screening for secretory phenotypes

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