Porcine pancreatic ductal epithelial cells transformed with KRASG12D and SV40T are tumorigenic

Abstract We describe our initial studies in the development of an orthotopic, genetically defined, large animal model of pancreatic cancer. Primary pancreatic epithelial cells were isolated from pancreatic duct of domestic pigs. A transformed cell line was generated from these primary cells with onc...

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Autores principales:	Katie L. Bailey, Sara B. Cartwright, Neesha S. Patel, Neeley Remmers, Audrey J. Lazenby, Michael A. Hollingsworth, Mark A. Carlson
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7af2948a4821425da7bdc200f1faf33e
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spelling	oai:doaj.org-article:7af2948a4821425da7bdc200f1faf33e2021-12-02T18:18:51ZPorcine pancreatic ductal epithelial cells transformed with KRASG12D and SV40T are tumorigenic10.1038/s41598-021-92852-22045-2322https://doaj.org/article/7af2948a4821425da7bdc200f1faf33e2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92852-2https://doaj.org/toc/2045-2322Abstract We describe our initial studies in the development of an orthotopic, genetically defined, large animal model of pancreatic cancer. Primary pancreatic epithelial cells were isolated from pancreatic duct of domestic pigs. A transformed cell line was generated from these primary cells with oncogenic KRAS and SV40T. The transformed cell lines outperformed the primary and SV40T immortalized cells in terms of proliferation, population doubling time, soft agar growth, transwell migration and invasion. The transformed cell line grew tumors when injected subcutaneously in nude mice, forming glandular structures and staining for epithelial markers. Future work will include implantation studies of these tumorigenic porcine pancreatic cell lines into the pancreas of allogeneic and autologous pigs. The resultant large animal model of pancreatic cancer could be utilized for preclinical research on diagnostic, interventional, and therapeutic technologies.Katie L. BaileySara B. CartwrightNeesha S. PatelNeeley RemmersAudrey J. LazenbyMichael A. HollingsworthMark A. CarlsonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Katie L. Bailey Sara B. Cartwright Neesha S. Patel Neeley Remmers Audrey J. Lazenby Michael A. Hollingsworth Mark A. Carlson Porcine pancreatic ductal epithelial cells transformed with KRASG12D and SV40T are tumorigenic
description	Abstract We describe our initial studies in the development of an orthotopic, genetically defined, large animal model of pancreatic cancer. Primary pancreatic epithelial cells were isolated from pancreatic duct of domestic pigs. A transformed cell line was generated from these primary cells with oncogenic KRAS and SV40T. The transformed cell lines outperformed the primary and SV40T immortalized cells in terms of proliferation, population doubling time, soft agar growth, transwell migration and invasion. The transformed cell line grew tumors when injected subcutaneously in nude mice, forming glandular structures and staining for epithelial markers. Future work will include implantation studies of these tumorigenic porcine pancreatic cell lines into the pancreas of allogeneic and autologous pigs. The resultant large animal model of pancreatic cancer could be utilized for preclinical research on diagnostic, interventional, and therapeutic technologies.
format	article
author	Katie L. Bailey Sara B. Cartwright Neesha S. Patel Neeley Remmers Audrey J. Lazenby Michael A. Hollingsworth Mark A. Carlson
author_facet	Katie L. Bailey Sara B. Cartwright Neesha S. Patel Neeley Remmers Audrey J. Lazenby Michael A. Hollingsworth Mark A. Carlson
author_sort	Katie L. Bailey
title	Porcine pancreatic ductal epithelial cells transformed with KRASG12D and SV40T are tumorigenic
title_short	Porcine pancreatic ductal epithelial cells transformed with KRASG12D and SV40T are tumorigenic
title_full	Porcine pancreatic ductal epithelial cells transformed with KRASG12D and SV40T are tumorigenic
title_fullStr	Porcine pancreatic ductal epithelial cells transformed with KRASG12D and SV40T are tumorigenic
title_full_unstemmed	Porcine pancreatic ductal epithelial cells transformed with KRASG12D and SV40T are tumorigenic
title_sort	porcine pancreatic ductal epithelial cells transformed with krasg12d and sv40t are tumorigenic
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7af2948a4821425da7bdc200f1faf33e
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Porcine pancreatic ductal epithelial cells transformed with KRASG12D and SV40T are tumorigenic

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