Using nanoBRET and CRISPR/Cas9 to monitor proximity to a genome-edited protein in real-time
Abstract Bioluminescence resonance energy transfer (BRET) has been a vital tool for understanding G protein-coupled receptor (GPCR) function. It has been used to investigate GPCR-protein and/or -ligand interactions as well as GPCR oligomerisation. However the utility of BRET is limited by the requir...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | article |
| Language: | EN |
| Published: |
Nature Portfolio
2017
|
| Subjects: | |
| Online Access: | https://doaj.org/article/7b3fcf6037e049f69f6a0bb8d24be1b0 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|