ORBIT: a New Paradigm for Genetic Engineering of Mycobacterial Chromosomes
ABSTRACT Two efficient recombination systems were combined to produce a versatile method for chromosomal engineering that obviates the need to prepare double-stranded DNA (dsDNA) recombination substrates. A synthetic “targeting oligonucleotide” is incorporated into the chromosome via homologous reco...
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| Main Authors: | , , , , , |
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| Format: | article |
| Language: | EN |
| Published: |
American Society for Microbiology
2018
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| Subjects: | |
| Online Access: | https://doaj.org/article/7bcb391be5a34a75b7fca29da7780f2c |
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