Template activating factor-I epigenetically regulates the TERT transcription in human cancer cells

Abstract Telomere, the terminus of linear chromosome in eukaryotes, is composed of specific repeat DNA which is mainly synthesized by a protein complex called telomerase. The maintenance of telomere DNA is important for unlimited proliferative capacity of cancer cells. The telomerase activity is con...

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Autores principales:	Kohsuke Kato, Atsushi Kawaguchi, Kyosuke Nagata
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7d5e3641ed684d32bda976d4bba12503
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spelling	oai:doaj.org-article:7d5e3641ed684d32bda976d4bba125032021-12-02T17:19:15ZTemplate activating factor-I epigenetically regulates the TERT transcription in human cancer cells10.1038/s41598-021-97009-92045-2322https://doaj.org/article/7d5e3641ed684d32bda976d4bba125032021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97009-9https://doaj.org/toc/2045-2322Abstract Telomere, the terminus of linear chromosome in eukaryotes, is composed of specific repeat DNA which is mainly synthesized by a protein complex called telomerase. The maintenance of telomere DNA is important for unlimited proliferative capacity of cancer cells. The telomerase activity is controlled by the expression level of telomerase reverse transcriptase (TERT), a catalytic unit of telomerase, in some species including human. Therefore, to reveal the regulatory mechanisms of the transcription of TERT gene is important for understanding the tumor development. We found that template activating factor-I (TAF-I), a multifunctional nuclear protein, is involved in the transcriptional activation of TERT for the maintenance of telomere DNA in HeLa cells. TAF-I maintains the histone H3 modifications involved in transcriptional activation and hypomethylated cytosines in CpG dinucleotides around the transcription start site (TSS) in the TERT gene locus. Collectively, TAF-I is involved in the maintenance of telomere DNA through the regulation of TERT transcription, then consequently the occurrence and/or recurrence of cancer cells.Kohsuke KatoAtsushi KawaguchiKyosuke NagataNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kohsuke Kato Atsushi Kawaguchi Kyosuke Nagata Template activating factor-I epigenetically regulates the TERT transcription in human cancer cells
description	Abstract Telomere, the terminus of linear chromosome in eukaryotes, is composed of specific repeat DNA which is mainly synthesized by a protein complex called telomerase. The maintenance of telomere DNA is important for unlimited proliferative capacity of cancer cells. The telomerase activity is controlled by the expression level of telomerase reverse transcriptase (TERT), a catalytic unit of telomerase, in some species including human. Therefore, to reveal the regulatory mechanisms of the transcription of TERT gene is important for understanding the tumor development. We found that template activating factor-I (TAF-I), a multifunctional nuclear protein, is involved in the transcriptional activation of TERT for the maintenance of telomere DNA in HeLa cells. TAF-I maintains the histone H3 modifications involved in transcriptional activation and hypomethylated cytosines in CpG dinucleotides around the transcription start site (TSS) in the TERT gene locus. Collectively, TAF-I is involved in the maintenance of telomere DNA through the regulation of TERT transcription, then consequently the occurrence and/or recurrence of cancer cells.
format	article
author	Kohsuke Kato Atsushi Kawaguchi Kyosuke Nagata
author_facet	Kohsuke Kato Atsushi Kawaguchi Kyosuke Nagata
author_sort	Kohsuke Kato
title	Template activating factor-I epigenetically regulates the TERT transcription in human cancer cells
title_short	Template activating factor-I epigenetically regulates the TERT transcription in human cancer cells
title_full	Template activating factor-I epigenetically regulates the TERT transcription in human cancer cells
title_fullStr	Template activating factor-I epigenetically regulates the TERT transcription in human cancer cells
title_full_unstemmed	Template activating factor-I epigenetically regulates the TERT transcription in human cancer cells
title_sort	template activating factor-i epigenetically regulates the tert transcription in human cancer cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7d5e3641ed684d32bda976d4bba12503
work_keys_str_mv	AT kohsukekato templateactivatingfactoriepigeneticallyregulatestheterttranscriptioninhumancancercells AT atsushikawaguchi templateactivatingfactoriepigeneticallyregulatestheterttranscriptioninhumancancercells AT kyosukenagata templateactivatingfactoriepigeneticallyregulatestheterttranscriptioninhumancancercells
_version_	1718381034925457408

Template activating factor-I epigenetically regulates the TERT transcription in human cancer cells

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