Improved fermentation efficiency of S. cerevisiae by changing glycolytic metabolic pathways with plasma agitation

Abstract Production of ethanol by the yeast Saccharomyces cerevisiae is a process of global importance. In these processes, productivities and yields are pushed to their maximum possible values leading to cellular stress. Transient and lasting enhancements in tolerance and performance have been obta...

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Autores principales: Nina Recek, Renwu Zhou, Rusen Zhou, Valentino Setoa Junior Te’o, Robert E. Speight, Miran Mozetič, Alenka Vesel, Uros Cvelbar, Kateryna Bazaka, Kostya (Ken) Ostrikov
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Publicado: Nature Portfolio 2018
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Acceso en línea:https://doaj.org/article/7d91fd9a10ce4b1e9b75a357f5d73313
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spelling oai:doaj.org-article:7d91fd9a10ce4b1e9b75a357f5d733132021-12-02T11:40:16ZImproved fermentation efficiency of S. cerevisiae by changing glycolytic metabolic pathways with plasma agitation10.1038/s41598-018-26227-52045-2322https://doaj.org/article/7d91fd9a10ce4b1e9b75a357f5d733132018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-26227-5https://doaj.org/toc/2045-2322Abstract Production of ethanol by the yeast Saccharomyces cerevisiae is a process of global importance. In these processes, productivities and yields are pushed to their maximum possible values leading to cellular stress. Transient and lasting enhancements in tolerance and performance have been obtained by genetic engineering, forced evolution, and exposure to moderate levels of chemical and/or physical stimuli, yet the drawbacks of these methods include cost, and multi-step, complex and lengthy treatment protocols. Here, plasma agitation is shown to rapidly induce desirable phenotypic changes in S. cerevisiae after a single treatment, resulting in improved conversion of glucose to ethanol. With a complex environment rich in energetic electrons, highly-reactive chemical species, photons, and gas flow effects, plasma treatment simultaneously mimics exposure to multiple environmental stressors. A single treatment of up to 10 minutes performed using an atmospheric pressure plasma jet was sufficient to induce changes in cell membrane structure, and increased hexokinase 2 activity and secondary metabolite production. These results suggest that plasma treatment is a promising strategy that can contribute to improving metabolic activity in industrial microbial strains, and thus the practicality and economics of industrial fermentations.Nina RecekRenwu ZhouRusen ZhouValentino Setoa Junior Te’oRobert E. SpeightMiran MozetičAlenka VeselUros CvelbarKateryna BazakaKostya (Ken) OstrikovNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-13 (2018)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Nina Recek
Renwu Zhou
Rusen Zhou
Valentino Setoa Junior Te’o
Robert E. Speight
Miran Mozetič
Alenka Vesel
Uros Cvelbar
Kateryna Bazaka
Kostya (Ken) Ostrikov
Improved fermentation efficiency of S. cerevisiae by changing glycolytic metabolic pathways with plasma agitation
description Abstract Production of ethanol by the yeast Saccharomyces cerevisiae is a process of global importance. In these processes, productivities and yields are pushed to their maximum possible values leading to cellular stress. Transient and lasting enhancements in tolerance and performance have been obtained by genetic engineering, forced evolution, and exposure to moderate levels of chemical and/or physical stimuli, yet the drawbacks of these methods include cost, and multi-step, complex and lengthy treatment protocols. Here, plasma agitation is shown to rapidly induce desirable phenotypic changes in S. cerevisiae after a single treatment, resulting in improved conversion of glucose to ethanol. With a complex environment rich in energetic electrons, highly-reactive chemical species, photons, and gas flow effects, plasma treatment simultaneously mimics exposure to multiple environmental stressors. A single treatment of up to 10 minutes performed using an atmospheric pressure plasma jet was sufficient to induce changes in cell membrane structure, and increased hexokinase 2 activity and secondary metabolite production. These results suggest that plasma treatment is a promising strategy that can contribute to improving metabolic activity in industrial microbial strains, and thus the practicality and economics of industrial fermentations.
format article
author Nina Recek
Renwu Zhou
Rusen Zhou
Valentino Setoa Junior Te’o
Robert E. Speight
Miran Mozetič
Alenka Vesel
Uros Cvelbar
Kateryna Bazaka
Kostya (Ken) Ostrikov
author_facet Nina Recek
Renwu Zhou
Rusen Zhou
Valentino Setoa Junior Te’o
Robert E. Speight
Miran Mozetič
Alenka Vesel
Uros Cvelbar
Kateryna Bazaka
Kostya (Ken) Ostrikov
author_sort Nina Recek
title Improved fermentation efficiency of S. cerevisiae by changing glycolytic metabolic pathways with plasma agitation
title_short Improved fermentation efficiency of S. cerevisiae by changing glycolytic metabolic pathways with plasma agitation
title_full Improved fermentation efficiency of S. cerevisiae by changing glycolytic metabolic pathways with plasma agitation
title_fullStr Improved fermentation efficiency of S. cerevisiae by changing glycolytic metabolic pathways with plasma agitation
title_full_unstemmed Improved fermentation efficiency of S. cerevisiae by changing glycolytic metabolic pathways with plasma agitation
title_sort improved fermentation efficiency of s. cerevisiae by changing glycolytic metabolic pathways with plasma agitation
publisher Nature Portfolio
publishDate 2018
url https://doaj.org/article/7d91fd9a10ce4b1e9b75a357f5d73313
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