Improved fermentation efficiency of S. cerevisiae by changing glycolytic metabolic pathways with plasma agitation

Abstract Production of ethanol by the yeast Saccharomyces cerevisiae is a process of global importance. In these processes, productivities and yields are pushed to their maximum possible values leading to cellular stress. Transient and lasting enhancements in tolerance and performance have been obta...

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Main Authors:	Nina Recek, Renwu Zhou, Rusen Zhou, Valentino Setoa Junior Te’o, Robert E. Speight, Miran Mozetič, Alenka Vesel, Uros Cvelbar, Kateryna Bazaka, Kostya (Ken) Ostrikov
Format:	article
Language:	EN
Published:	Nature Portfolio 2018
Subjects:	Medicine R Science Q
Online Access:	https://doaj.org/article/7d91fd9a10ce4b1e9b75a357f5d73313
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spelling	oai:doaj.org-article:7d91fd9a10ce4b1e9b75a357f5d733132021-12-02T11:40:16ZImproved fermentation efficiency of S. cerevisiae by changing glycolytic metabolic pathways with plasma agitation10.1038/s41598-018-26227-52045-2322https://doaj.org/article/7d91fd9a10ce4b1e9b75a357f5d733132018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-26227-5https://doaj.org/toc/2045-2322Abstract Production of ethanol by the yeast Saccharomyces cerevisiae is a process of global importance. In these processes, productivities and yields are pushed to their maximum possible values leading to cellular stress. Transient and lasting enhancements in tolerance and performance have been obtained by genetic engineering, forced evolution, and exposure to moderate levels of chemical and/or physical stimuli, yet the drawbacks of these methods include cost, and multi-step, complex and lengthy treatment protocols. Here, plasma agitation is shown to rapidly induce desirable phenotypic changes in S. cerevisiae after a single treatment, resulting in improved conversion of glucose to ethanol. With a complex environment rich in energetic electrons, highly-reactive chemical species, photons, and gas flow effects, plasma treatment simultaneously mimics exposure to multiple environmental stressors. A single treatment of up to 10 minutes performed using an atmospheric pressure plasma jet was sufficient to induce changes in cell membrane structure, and increased hexokinase 2 activity and secondary metabolite production. These results suggest that plasma treatment is a promising strategy that can contribute to improving metabolic activity in industrial microbial strains, and thus the practicality and economics of industrial fermentations.Nina RecekRenwu ZhouRusen ZhouValentino Setoa Junior Te’oRobert E. SpeightMiran MozetičAlenka VeselUros CvelbarKateryna BazakaKostya (Ken) OstrikovNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-13 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nina Recek Renwu Zhou Rusen Zhou Valentino Setoa Junior Te’o Robert E. Speight Miran Mozetič Alenka Vesel Uros Cvelbar Kateryna Bazaka Kostya (Ken) Ostrikov Improved fermentation efficiency of S. cerevisiae by changing glycolytic metabolic pathways with plasma agitation
description	Abstract Production of ethanol by the yeast Saccharomyces cerevisiae is a process of global importance. In these processes, productivities and yields are pushed to their maximum possible values leading to cellular stress. Transient and lasting enhancements in tolerance and performance have been obtained by genetic engineering, forced evolution, and exposure to moderate levels of chemical and/or physical stimuli, yet the drawbacks of these methods include cost, and multi-step, complex and lengthy treatment protocols. Here, plasma agitation is shown to rapidly induce desirable phenotypic changes in S. cerevisiae after a single treatment, resulting in improved conversion of glucose to ethanol. With a complex environment rich in energetic electrons, highly-reactive chemical species, photons, and gas flow effects, plasma treatment simultaneously mimics exposure to multiple environmental stressors. A single treatment of up to 10 minutes performed using an atmospheric pressure plasma jet was sufficient to induce changes in cell membrane structure, and increased hexokinase 2 activity and secondary metabolite production. These results suggest that plasma treatment is a promising strategy that can contribute to improving metabolic activity in industrial microbial strains, and thus the practicality and economics of industrial fermentations.
format	article
author	Nina Recek Renwu Zhou Rusen Zhou Valentino Setoa Junior Te’o Robert E. Speight Miran Mozetič Alenka Vesel Uros Cvelbar Kateryna Bazaka Kostya (Ken) Ostrikov
author_facet	Nina Recek Renwu Zhou Rusen Zhou Valentino Setoa Junior Te’o Robert E. Speight Miran Mozetič Alenka Vesel Uros Cvelbar Kateryna Bazaka Kostya (Ken) Ostrikov
author_sort	Nina Recek
title	Improved fermentation efficiency of S. cerevisiae by changing glycolytic metabolic pathways with plasma agitation
title_short	Improved fermentation efficiency of S. cerevisiae by changing glycolytic metabolic pathways with plasma agitation
title_full	Improved fermentation efficiency of S. cerevisiae by changing glycolytic metabolic pathways with plasma agitation
title_fullStr	Improved fermentation efficiency of S. cerevisiae by changing glycolytic metabolic pathways with plasma agitation
title_full_unstemmed	Improved fermentation efficiency of S. cerevisiae by changing glycolytic metabolic pathways with plasma agitation
title_sort	improved fermentation efficiency of s. cerevisiae by changing glycolytic metabolic pathways with plasma agitation
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/7d91fd9a10ce4b1e9b75a357f5d73313
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Improved fermentation efficiency of S. cerevisiae by changing glycolytic metabolic pathways with plasma agitation

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