Matrilin3/TGFβ3 gelatin microparticles promote chondrogenesis, prevent hypertrophy, and induce paracrine release in MSC spheroid for disc regeneration

Abstract Degenerative disc disease (DDD) is the leading cause of excruciating lower back pain and disability in adults worldwide. Among the current treatments for DDD, cell-based therapies such as the injection of both disc- and non-disc-derived chondrocytes have shown significant improvements in th...

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Autores principales:	Alvin Bacero Bello, Yunkyung Kim, Sunghyun Park, Manjunatha S. Muttigi, Jiseong Kim, Hansoo Park, Soohong Lee
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R
Acceso en línea:	https://doaj.org/article/7de8c9d1ebed42328e651f3ec0bbee73
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spelling	oai:doaj.org-article:7de8c9d1ebed42328e651f3ec0bbee732021-12-02T16:38:27ZMatrilin3/TGFβ3 gelatin microparticles promote chondrogenesis, prevent hypertrophy, and induce paracrine release in MSC spheroid for disc regeneration10.1038/s41536-021-00160-02057-3995https://doaj.org/article/7de8c9d1ebed42328e651f3ec0bbee732021-09-01T00:00:00Zhttps://doi.org/10.1038/s41536-021-00160-0https://doaj.org/toc/2057-3995Abstract Degenerative disc disease (DDD) is the leading cause of excruciating lower back pain and disability in adults worldwide. Among the current treatments for DDD, cell-based therapies such as the injection of both disc- and non-disc-derived chondrocytes have shown significant improvements in the patients’ condition. However, further advancement of these therapies is required to not only ensure a supply of healthy chondrocytes but also to promote regeneration of the defective cells in the injury site. Here, we report that the incorporation of gelatin microparticles coloaded with transforming growth factor beta 3 and matrilin 3 promoted chondrogenic differentiation of adipose-derived mesenchymal stem cell spheroids while preventing hypertrophy and terminal differentiation of cells. Moreover, these composite spheroids induced the release of chondrogenic cytokines that, in turn, promoted regeneration of degenerative chondrocytes in vitro. Finally, injections of these composite spheroids in a rat model of intervertebral disc disease promoted restoration of the chondrogenic properties of the cells, thereby allowing regeneration of the chondrogenic tissue in vivo.Alvin Bacero BelloYunkyung KimSunghyun ParkManjunatha S. MuttigiJiseong KimHansoo ParkSoohong LeeNature PortfolioarticleMedicineRENnpj Regenerative Medicine, Vol 6, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R
spellingShingle	Medicine R Alvin Bacero Bello Yunkyung Kim Sunghyun Park Manjunatha S. Muttigi Jiseong Kim Hansoo Park Soohong Lee Matrilin3/TGFβ3 gelatin microparticles promote chondrogenesis, prevent hypertrophy, and induce paracrine release in MSC spheroid for disc regeneration
description	Abstract Degenerative disc disease (DDD) is the leading cause of excruciating lower back pain and disability in adults worldwide. Among the current treatments for DDD, cell-based therapies such as the injection of both disc- and non-disc-derived chondrocytes have shown significant improvements in the patients’ condition. However, further advancement of these therapies is required to not only ensure a supply of healthy chondrocytes but also to promote regeneration of the defective cells in the injury site. Here, we report that the incorporation of gelatin microparticles coloaded with transforming growth factor beta 3 and matrilin 3 promoted chondrogenic differentiation of adipose-derived mesenchymal stem cell spheroids while preventing hypertrophy and terminal differentiation of cells. Moreover, these composite spheroids induced the release of chondrogenic cytokines that, in turn, promoted regeneration of degenerative chondrocytes in vitro. Finally, injections of these composite spheroids in a rat model of intervertebral disc disease promoted restoration of the chondrogenic properties of the cells, thereby allowing regeneration of the chondrogenic tissue in vivo.
format	article
author	Alvin Bacero Bello Yunkyung Kim Sunghyun Park Manjunatha S. Muttigi Jiseong Kim Hansoo Park Soohong Lee
author_facet	Alvin Bacero Bello Yunkyung Kim Sunghyun Park Manjunatha S. Muttigi Jiseong Kim Hansoo Park Soohong Lee
author_sort	Alvin Bacero Bello
title	Matrilin3/TGFβ3 gelatin microparticles promote chondrogenesis, prevent hypertrophy, and induce paracrine release in MSC spheroid for disc regeneration
title_short	Matrilin3/TGFβ3 gelatin microparticles promote chondrogenesis, prevent hypertrophy, and induce paracrine release in MSC spheroid for disc regeneration
title_full	Matrilin3/TGFβ3 gelatin microparticles promote chondrogenesis, prevent hypertrophy, and induce paracrine release in MSC spheroid for disc regeneration
title_fullStr	Matrilin3/TGFβ3 gelatin microparticles promote chondrogenesis, prevent hypertrophy, and induce paracrine release in MSC spheroid for disc regeneration
title_full_unstemmed	Matrilin3/TGFβ3 gelatin microparticles promote chondrogenesis, prevent hypertrophy, and induce paracrine release in MSC spheroid for disc regeneration
title_sort	matrilin3/tgfβ3 gelatin microparticles promote chondrogenesis, prevent hypertrophy, and induce paracrine release in msc spheroid for disc regeneration
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7de8c9d1ebed42328e651f3ec0bbee73
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Matrilin3/TGFβ3 gelatin microparticles promote chondrogenesis, prevent hypertrophy, and induce paracrine release in MSC spheroid for disc regeneration

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