High-intensity UV laser ChIP-seq for the study of protein-DNA interactions in living cells

Chromatin immunoprecipitation followed by DNA sequencing (ChIP-seq) can map transcription factor binding to a given genome. Here, Steube and colleagues devise a ChIP-seq technique based on photochemical crosslinking by high-intensity ultraviolet laser irradiation, and describe its utility.

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Bibliographic Details
Main Authors: Arndt Steube, Tino Schenk, Alexander Tretyakov, Hans Peter Saluz
Format: article
Language:EN
Published: Nature Portfolio 2017
Subjects:
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Online Access:https://doaj.org/article/8044421ab89d460e975d3817b5e10749
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