Genome-wide microhomologies enable precise template-free editing of biologically relevant deletion mutations

Genome-wide microhomologies enable precise template-free editing of biologically relevant deletion mutations

DNA repair by microhomology-mediated end joining creates precise deletions based on flanking microhomologies. Here the authors use CRISPR-Cas9 to recreate pathogenic deletion mutations using existing microhomologies in the human genome identified by their program MHcut.

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Bibliographic Details
Main Authors: Janin Grajcarek, Jean Monlong, Yoko Nishinaka-Arai, Michiko Nakamura, Miki Nagai, Shiori Matsuo, David Lougheed, Hidetoshi Sakurai, Megumu K. Saito, Guillaume Bourque, Knut Woltjen
Format: article
Language:EN
Published: Nature Portfolio 2019
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Science
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Online Access:https://doaj.org/article/83dd1a923e3149b5847e613384f3d67a
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Summary:DNA repair by microhomology-mediated end joining creates precise deletions based on flanking microhomologies. Here the authors use CRISPR-Cas9 to recreate pathogenic deletion mutations using existing microhomologies in the human genome identified by their program MHcut.

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