Determining biophysical protein stability in lysates by a fast proteolysis assay, FASTpp.
The biophysical stability is an important parameter for protein activity both in vivo and in vitro. Here we propose a method to analyse thermal melting of protein domains in lysates: Fast parallel proteolysis (FASTpp). Combining unfolding by a temperature gradient in a thermal cycler with simultaneo...
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| Auteurs principaux: | , , |
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| Format: | article |
| Langue: | EN |
| Publié: |
Public Library of Science (PLoS)
2012
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| Accès en ligne: | https://doaj.org/article/8434dd8b31c14f2597496c289286d9e6 |
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