Determining biophysical protein stability in lysates by a fast proteolysis assay, FASTpp.

The biophysical stability is an important parameter for protein activity both in vivo and in vitro. Here we propose a method to analyse thermal melting of protein domains in lysates: Fast parallel proteolysis (FASTpp). Combining unfolding by a temperature gradient in a thermal cycler with simultaneo...

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Bibliographic Details
Main Authors: David P Minde, Madelon M Maurice, Stefan G D Rüdiger
Format: article
Language:EN
Published: Public Library of Science (PLoS) 2012
Subjects:
R
Q
Online Access:https://doaj.org/article/8434dd8b31c14f2597496c289286d9e6
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