Garlic Extract: Inhibition of Biochemical and Biophysical Changes in Glycated HSA

Glycation of various biomolecules contributes to structural changes and formation of several high molecular weight fluorescent and non-fluorescent, advanced glycation end products (AGEs). AGEs and glycation are involved in various health complications. Synthetic medicines, including metformin, have...

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Autores principales:	Mohd W. A. Khan, Ahmed A. Otaibi, Arwa F. M. Alhumaid, Abdulmohsen K. D. Alsukaibi, Asma K. Alshamari, Eida M. Alshammari, Salma A. Al-Zahrani, Ahmed Y. M. Almudyani, Subuhi Sherwani
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	glycation natural products garlic extract biochemical biophysical HSA Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/84929fc583bd4b46aba8b58e7a16f818
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spelling	oai:doaj.org-article:84929fc583bd4b46aba8b58e7a16f8182021-11-25T16:43:06ZGarlic Extract: Inhibition of Biochemical and Biophysical Changes in Glycated HSA10.3390/app1122110282076-3417https://doaj.org/article/84929fc583bd4b46aba8b58e7a16f8182021-11-01T00:00:00Zhttps://www.mdpi.com/2076-3417/11/22/11028https://doaj.org/toc/2076-3417Glycation of various biomolecules contributes to structural changes and formation of several high molecular weight fluorescent and non-fluorescent, advanced glycation end products (AGEs). AGEs and glycation are involved in various health complications. Synthetic medicines, including metformin, have several adverse effects. Natural products and their derivatives are used in the treatment of various diseases due to their significant therapeutic qualities. <i>Allium sativum</i> (garlic) is used in traditional medicines because of its antioxidant, anti-inflammatory, and anti-diabetic properties. This study aimed to determine the anti-glycating and AGEs inhibitory activities of garlic. Biochemical and biophysical analyses were performed for in vitro incubated human serum albumin (HSA) with 0.05 M of glucose for 1, 5, and 10 weeks. Anti-glycating and AGEs inhibitory effect of garlic was investigated in glycated samples. Increased biochemical and biophysical changes were observed in glycated HSA incubated for 10 weeks (G-HSA-10W) as compared to native HSA (N-HSA) as well as glycated HSA incubated for 1 (G-HSA-1W) and 5 weeks (G-HSA-5W). Garlic extract with a concentration of ≥6.25 µg/mL exhibited significant inhibition in biophysical and biochemical changes of G-HSA-10W. Our findings demonstrated that garlic extract has the ability to inhibit biochemical and biophysical changes in HSA that occurred due to glycation. Thus, garlic extract can be used against glycation and AGE-related health complications linked with chronic diseases in diabetic patients due to its broad therapeutic potential.Mohd W. A. KhanAhmed A. OtaibiArwa F. M. AlhumaidAbdulmohsen K. D. AlsukaibiAsma K. AlshamariEida M. AlshammariSalma A. Al-ZahraniAhmed Y. M. AlmudyaniSubuhi SherwaniMDPI AGarticleglycationnatural productsgarlic extractbiochemicalbiophysicalHSATechnologyTEngineering (General). Civil engineering (General)TA1-2040Biology (General)QH301-705.5PhysicsQC1-999ChemistryQD1-999ENApplied Sciences, Vol 11, Iss 11028, p 11028 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	glycation natural products garlic extract biochemical biophysical HSA Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999
spellingShingle	glycation natural products garlic extract biochemical biophysical HSA Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999 Mohd W. A. Khan Ahmed A. Otaibi Arwa F. M. Alhumaid Abdulmohsen K. D. Alsukaibi Asma K. Alshamari Eida M. Alshammari Salma A. Al-Zahrani Ahmed Y. M. Almudyani Subuhi Sherwani Garlic Extract: Inhibition of Biochemical and Biophysical Changes in Glycated HSA
description	Glycation of various biomolecules contributes to structural changes and formation of several high molecular weight fluorescent and non-fluorescent, advanced glycation end products (AGEs). AGEs and glycation are involved in various health complications. Synthetic medicines, including metformin, have several adverse effects. Natural products and their derivatives are used in the treatment of various diseases due to their significant therapeutic qualities. <i>Allium sativum</i> (garlic) is used in traditional medicines because of its antioxidant, anti-inflammatory, and anti-diabetic properties. This study aimed to determine the anti-glycating and AGEs inhibitory activities of garlic. Biochemical and biophysical analyses were performed for in vitro incubated human serum albumin (HSA) with 0.05 M of glucose for 1, 5, and 10 weeks. Anti-glycating and AGEs inhibitory effect of garlic was investigated in glycated samples. Increased biochemical and biophysical changes were observed in glycated HSA incubated for 10 weeks (G-HSA-10W) as compared to native HSA (N-HSA) as well as glycated HSA incubated for 1 (G-HSA-1W) and 5 weeks (G-HSA-5W). Garlic extract with a concentration of ≥6.25 µg/mL exhibited significant inhibition in biophysical and biochemical changes of G-HSA-10W. Our findings demonstrated that garlic extract has the ability to inhibit biochemical and biophysical changes in HSA that occurred due to glycation. Thus, garlic extract can be used against glycation and AGE-related health complications linked with chronic diseases in diabetic patients due to its broad therapeutic potential.
format	article
author	Mohd W. A. Khan Ahmed A. Otaibi Arwa F. M. Alhumaid Abdulmohsen K. D. Alsukaibi Asma K. Alshamari Eida M. Alshammari Salma A. Al-Zahrani Ahmed Y. M. Almudyani Subuhi Sherwani
author_facet	Mohd W. A. Khan Ahmed A. Otaibi Arwa F. M. Alhumaid Abdulmohsen K. D. Alsukaibi Asma K. Alshamari Eida M. Alshammari Salma A. Al-Zahrani Ahmed Y. M. Almudyani Subuhi Sherwani
author_sort	Mohd W. A. Khan
title	Garlic Extract: Inhibition of Biochemical and Biophysical Changes in Glycated HSA
title_short	Garlic Extract: Inhibition of Biochemical and Biophysical Changes in Glycated HSA
title_full	Garlic Extract: Inhibition of Biochemical and Biophysical Changes in Glycated HSA
title_fullStr	Garlic Extract: Inhibition of Biochemical and Biophysical Changes in Glycated HSA
title_full_unstemmed	Garlic Extract: Inhibition of Biochemical and Biophysical Changes in Glycated HSA
title_sort	garlic extract: inhibition of biochemical and biophysical changes in glycated hsa
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/84929fc583bd4b46aba8b58e7a16f818
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Garlic Extract: Inhibition of Biochemical and Biophysical Changes in Glycated HSA

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