Two-color in-resin CLEM of Epon-embedded cells using osmium resistant green and red fluorescent proteins

Abstract In-resin CLEM of Epon embedded samples can greatly simplify the correlation of fluorescent images with electron micrographs. The usefulness of this technique is limited at present by the low number of fluorescent proteins that resist CLEM processing. Additionally, no study has reported the...

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Bibliographic Details
Main Authors: Isei Tanida, Yoko Furuta, Junji Yamaguchi, Soichiro Kakuta, Juan Alejandro Oliva Trejo, Yasuo Uchiyama
Format: article
Language:EN
Published: Nature Portfolio 2020
Subjects:
R
Q
Online Access:https://doaj.org/article/84e16da512444879bfc9d6a0294bd7cd
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