MiR-191-5p alleviates microglial cell injury by targeting Map3k12 (mitogen-activated protein kinase kinase kinase 12) to inhibit the MAPK (mitogen-activated protein kinase) signaling pathway in Alzheimer’s disease: Running Title: Role of miR-191-5p in Alzheimer’s disease

Alzheimer’s disease (AD) is a progressive neurodegenerative disease. Multiple reports have elucidated that microRNAs are promising biomarkers for AD diagnosis and treatment. Herein, the effect of miR-191-5p on microglial cell injury and the underlying mechanism were explored. APP/PS1 transgenic mice...

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Autores principales:	Wenjun Wan, Ganzhe Liu, Xia Li, Yu Liu, Ying Wang, Haisong Pan, Jun Hu
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	alzheimer’s disease mir-191-5p map3k12 microglia mapk signaling Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/86808bc2de7e4cd59af49aca62e20d8a
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spelling	oai:doaj.org-article:86808bc2de7e4cd59af49aca62e20d8a2021-11-26T11:19:50ZMiR-191-5p alleviates microglial cell injury by targeting Map3k12 (mitogen-activated protein kinase kinase kinase 12) to inhibit the MAPK (mitogen-activated protein kinase) signaling pathway in Alzheimer’s disease: Running Title: Role of miR-191-5p in Alzheimer’s disease2165-59792165-598710.1080/21655979.2021.2008638https://doaj.org/article/86808bc2de7e4cd59af49aca62e20d8a2021-11-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.2008638https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Alzheimer’s disease (AD) is a progressive neurodegenerative disease. Multiple reports have elucidated that microRNAs are promising biomarkers for AD diagnosis and treatment. Herein, the effect of miR-191-5p on microglial cell injury and the underlying mechanism were explored. APP/PS1 transgenic mice were utilized to establish mouse model of AD. Amyloid-β protein 1-42 (Aβ1-42)-treated microglia were applied to establish in vitro cell model of AD. MiR-191-5p expression in hippocampus and microglia was measured by reverse transcription quantitative polymerase chain reaction. The viability and apoptosis of microglia were evaluated by Cell Counting Kit-8 assays and flow cytometry analyses, respectively. Binding relationship between miR-191-5p and its downstream target mitogen-activated protein kinase kinase kinase 12 (Map3k12) was determined by luciferase reporter assays. Pathological degeneration of hippocampus was tested using hematoxylin-eosin staining and Nissl staining. Aβ expression in hippocampus was examined via immunohistochemistry. In this study, miR-191-5p was downregulated in Aβ1-42-stimulated microglia and hippocampal tissues of APP/PS1 mice. MiR-191-5p overexpression facilitated cell viability and inhibited apoptosis rate of Aβ1-42-treated microglia. Mechanically, miR-191-5p targeted Map3k12 3ʹ-untranslated region to downregulate Map3k12 expression. MiR-191-5p inhibited Aβ1-42-induced microglial cell injury and inactivated the MAPK signaling by downregulating Map3k12. Overall, miR-191-5p alleviated Aβ1-42-induced microglia cell injury by targeting Map3k12 to inhibit the MAPK signaling pathway in microglia.Wenjun WanGanzhe LiuXia LiYu LiuYing WangHaisong PanJun HuTaylor & Francis Grouparticlealzheimer’s diseasemir-191-5pmap3k12microgliamapk signalingBiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	alzheimer’s disease mir-191-5p map3k12 microglia mapk signaling Biotechnology TP248.13-248.65
spellingShingle	alzheimer’s disease mir-191-5p map3k12 microglia mapk signaling Biotechnology TP248.13-248.65 Wenjun Wan Ganzhe Liu Xia Li Yu Liu Ying Wang Haisong Pan Jun Hu MiR-191-5p alleviates microglial cell injury by targeting Map3k12 (mitogen-activated protein kinase kinase kinase 12) to inhibit the MAPK (mitogen-activated protein kinase) signaling pathway in Alzheimer’s disease: Running Title: Role of miR-191-5p in Alzheimer’s disease
description	Alzheimer’s disease (AD) is a progressive neurodegenerative disease. Multiple reports have elucidated that microRNAs are promising biomarkers for AD diagnosis and treatment. Herein, the effect of miR-191-5p on microglial cell injury and the underlying mechanism were explored. APP/PS1 transgenic mice were utilized to establish mouse model of AD. Amyloid-β protein 1-42 (Aβ1-42)-treated microglia were applied to establish in vitro cell model of AD. MiR-191-5p expression in hippocampus and microglia was measured by reverse transcription quantitative polymerase chain reaction. The viability and apoptosis of microglia were evaluated by Cell Counting Kit-8 assays and flow cytometry analyses, respectively. Binding relationship between miR-191-5p and its downstream target mitogen-activated protein kinase kinase kinase 12 (Map3k12) was determined by luciferase reporter assays. Pathological degeneration of hippocampus was tested using hematoxylin-eosin staining and Nissl staining. Aβ expression in hippocampus was examined via immunohistochemistry. In this study, miR-191-5p was downregulated in Aβ1-42-stimulated microglia and hippocampal tissues of APP/PS1 mice. MiR-191-5p overexpression facilitated cell viability and inhibited apoptosis rate of Aβ1-42-treated microglia. Mechanically, miR-191-5p targeted Map3k12 3ʹ-untranslated region to downregulate Map3k12 expression. MiR-191-5p inhibited Aβ1-42-induced microglial cell injury and inactivated the MAPK signaling by downregulating Map3k12. Overall, miR-191-5p alleviated Aβ1-42-induced microglia cell injury by targeting Map3k12 to inhibit the MAPK signaling pathway in microglia.
format	article
author	Wenjun Wan Ganzhe Liu Xia Li Yu Liu Ying Wang Haisong Pan Jun Hu
author_facet	Wenjun Wan Ganzhe Liu Xia Li Yu Liu Ying Wang Haisong Pan Jun Hu
author_sort	Wenjun Wan
title	MiR-191-5p alleviates microglial cell injury by targeting Map3k12 (mitogen-activated protein kinase kinase kinase 12) to inhibit the MAPK (mitogen-activated protein kinase) signaling pathway in Alzheimer’s disease: Running Title: Role of miR-191-5p in Alzheimer’s disease
title_short	MiR-191-5p alleviates microglial cell injury by targeting Map3k12 (mitogen-activated protein kinase kinase kinase 12) to inhibit the MAPK (mitogen-activated protein kinase) signaling pathway in Alzheimer’s disease: Running Title: Role of miR-191-5p in Alzheimer’s disease
title_full	MiR-191-5p alleviates microglial cell injury by targeting Map3k12 (mitogen-activated protein kinase kinase kinase 12) to inhibit the MAPK (mitogen-activated protein kinase) signaling pathway in Alzheimer’s disease: Running Title: Role of miR-191-5p in Alzheimer’s disease
title_fullStr	MiR-191-5p alleviates microglial cell injury by targeting Map3k12 (mitogen-activated protein kinase kinase kinase 12) to inhibit the MAPK (mitogen-activated protein kinase) signaling pathway in Alzheimer’s disease: Running Title: Role of miR-191-5p in Alzheimer’s disease
title_full_unstemmed	MiR-191-5p alleviates microglial cell injury by targeting Map3k12 (mitogen-activated protein kinase kinase kinase 12) to inhibit the MAPK (mitogen-activated protein kinase) signaling pathway in Alzheimer’s disease: Running Title: Role of miR-191-5p in Alzheimer’s disease
title_sort	mir-191-5p alleviates microglial cell injury by targeting map3k12 (mitogen-activated protein kinase kinase kinase 12) to inhibit the mapk (mitogen-activated protein kinase) signaling pathway in alzheimer’s disease: running title: role of mir-191-5p in alzheimer’s disease
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/86808bc2de7e4cd59af49aca62e20d8a
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_version_	1718409483773804544

MiR-191-5p alleviates microglial cell injury by targeting Map3k12 (mitogen-activated protein kinase kinase kinase 12) to inhibit the MAPK (mitogen-activated protein kinase) signaling pathway in Alzheimer’s disease: Running Title: Role of miR-191-5p in Alzheimer’s disease

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