The Orai-1 and STIM-1 complex controls human dendritic cell maturation.

Ca(2+) signaling plays an important role in the function of dendritic cells (DC), the professional antigen presenting cells. Here, we described the role of Calcium released activated (CRAC) channels in the maturation and cytokine secretion of human DC. Recent works identified STIM1 and Orai1 in huma...

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Autores principales:	Romain Félix, David Crottès, Anthony Delalande, Jérémy Fauconnier, Yvon Lebranchu, Jean-Yves Le Guennec, Florence Velge-Roussel
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/869032f1d1e048c2831b3102cc74b2e6
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spelling	oai:doaj.org-article:869032f1d1e048c2831b3102cc74b2e62021-11-18T07:45:13ZThe Orai-1 and STIM-1 complex controls human dendritic cell maturation.1932-620310.1371/journal.pone.0061595https://doaj.org/article/869032f1d1e048c2831b3102cc74b2e62013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23700407/?tool=EBIhttps://doaj.org/toc/1932-6203Ca(2+) signaling plays an important role in the function of dendritic cells (DC), the professional antigen presenting cells. Here, we described the role of Calcium released activated (CRAC) channels in the maturation and cytokine secretion of human DC. Recent works identified STIM1 and Orai1 in human T lymphocytes as essential for CRAC channel activation. We investigated Ca(2+) signaling in human DC maturation by imaging intracellular calcium signaling and pharmalogical inhibitors. The DC response to inflammatory mediators or PAMPs (Pathogen-associated molecular patterns) is due to a depletion of intracellular Ca(2+) stores that results in a store-operated Ca(2+) entry (SOCE). This Ca(2+) influx was inhibited by 2-APB and exhibited a Ca(2+)permeability similar to the CRAC (Calcium-Released Activated Calcium), found in T lymphocytes. Depending on the PAMPs used, SOCE profiles and amplitudes appeared different, suggesting the involvement of different CRAC channels. Using siRNAi, we identified the STIM1 and Orai1 protein complex as one of the main pathways for Ca(2+) entry for LPS- and TNF-α-induced maturation in DC. Cytokine secretions also seemed to be SOCE-dependent with profile differences depending on the maturating agents since IL-12 and IL10 secretions appeared highly sensitive to 2-APB whereas IFN-γ was less affected. Altogether, these results clearly demonstrate that human DC maturation and cytokine secretions depend on SOCE signaling involving STIM1 and Orai1 proteins.Romain FélixDavid CrottèsAnthony DelalandeJérémy FauconnierYvon LebranchuJean-Yves Le GuennecFlorence Velge-RousselPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e61595 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Romain Félix David Crottès Anthony Delalande Jérémy Fauconnier Yvon Lebranchu Jean-Yves Le Guennec Florence Velge-Roussel The Orai-1 and STIM-1 complex controls human dendritic cell maturation.
description	Ca(2+) signaling plays an important role in the function of dendritic cells (DC), the professional antigen presenting cells. Here, we described the role of Calcium released activated (CRAC) channels in the maturation and cytokine secretion of human DC. Recent works identified STIM1 and Orai1 in human T lymphocytes as essential for CRAC channel activation. We investigated Ca(2+) signaling in human DC maturation by imaging intracellular calcium signaling and pharmalogical inhibitors. The DC response to inflammatory mediators or PAMPs (Pathogen-associated molecular patterns) is due to a depletion of intracellular Ca(2+) stores that results in a store-operated Ca(2+) entry (SOCE). This Ca(2+) influx was inhibited by 2-APB and exhibited a Ca(2+)permeability similar to the CRAC (Calcium-Released Activated Calcium), found in T lymphocytes. Depending on the PAMPs used, SOCE profiles and amplitudes appeared different, suggesting the involvement of different CRAC channels. Using siRNAi, we identified the STIM1 and Orai1 protein complex as one of the main pathways for Ca(2+) entry for LPS- and TNF-α-induced maturation in DC. Cytokine secretions also seemed to be SOCE-dependent with profile differences depending on the maturating agents since IL-12 and IL10 secretions appeared highly sensitive to 2-APB whereas IFN-γ was less affected. Altogether, these results clearly demonstrate that human DC maturation and cytokine secretions depend on SOCE signaling involving STIM1 and Orai1 proteins.
format	article
author	Romain Félix David Crottès Anthony Delalande Jérémy Fauconnier Yvon Lebranchu Jean-Yves Le Guennec Florence Velge-Roussel
author_facet	Romain Félix David Crottès Anthony Delalande Jérémy Fauconnier Yvon Lebranchu Jean-Yves Le Guennec Florence Velge-Roussel
author_sort	Romain Félix
title	The Orai-1 and STIM-1 complex controls human dendritic cell maturation.
title_short	The Orai-1 and STIM-1 complex controls human dendritic cell maturation.
title_full	The Orai-1 and STIM-1 complex controls human dendritic cell maturation.
title_fullStr	The Orai-1 and STIM-1 complex controls human dendritic cell maturation.
title_full_unstemmed	The Orai-1 and STIM-1 complex controls human dendritic cell maturation.
title_sort	orai-1 and stim-1 complex controls human dendritic cell maturation.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/869032f1d1e048c2831b3102cc74b2e6
work_keys_str_mv	AT romainfelix theorai1andstim1complexcontrolshumandendriticcellmaturation AT davidcrottes theorai1andstim1complexcontrolshumandendriticcellmaturation AT anthonydelalande theorai1andstim1complexcontrolshumandendriticcellmaturation AT jeremyfauconnier theorai1andstim1complexcontrolshumandendriticcellmaturation AT yvonlebranchu theorai1andstim1complexcontrolshumandendriticcellmaturation AT jeanyvesleguennec theorai1andstim1complexcontrolshumandendriticcellmaturation AT florencevelgeroussel theorai1andstim1complexcontrolshumandendriticcellmaturation AT romainfelix orai1andstim1complexcontrolshumandendriticcellmaturation AT davidcrottes orai1andstim1complexcontrolshumandendriticcellmaturation AT anthonydelalande orai1andstim1complexcontrolshumandendriticcellmaturation AT jeremyfauconnier orai1andstim1complexcontrolshumandendriticcellmaturation AT yvonlebranchu orai1andstim1complexcontrolshumandendriticcellmaturation AT jeanyvesleguennec orai1andstim1complexcontrolshumandendriticcellmaturation AT florencevelgeroussel orai1andstim1complexcontrolshumandendriticcellmaturation
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The Orai-1 and STIM-1 complex controls human dendritic cell maturation.

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