Identification of the effector domain of biglycan that facilitates BMP-2 osteogenic function
Abstract We have reported that recombinant biglycan (BGN) core protein accelerates bone formation in vivo by enhancing bone morphogenetic protein (BMP)-2 function. The purpose of the present study was to identify the specific domain (“effector”) within the BGN core protein that facilitates BMP-2 ost...
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Nature Portfolio
2018
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oai:doaj.org-article:86b12ac6f51546d392392532a8bb8bf52021-12-02T12:32:22ZIdentification of the effector domain of biglycan that facilitates BMP-2 osteogenic function10.1038/s41598-018-25279-x2045-2322https://doaj.org/article/86b12ac6f51546d392392532a8bb8bf52018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25279-xhttps://doaj.org/toc/2045-2322Abstract We have reported that recombinant biglycan (BGN) core protein accelerates bone formation in vivo by enhancing bone morphogenetic protein (BMP)-2 function. The purpose of the present study was to identify the specific domain (“effector”) within the BGN core protein that facilitates BMP-2 osteogenic function. Thus, we generated various recombinant and synthetic peptides corresponding to several domains of BGN, and tested their effects on BMP-2 functions in vitro. The results demonstrated that the leucine-rich repeats 2–3 domain (LRR2-3) of BGN significantly enhanced the BMP-2 induced Smad1/5/9 phosphorylation, osteogenic gene expression, and alkaline phosphatase activity in myogenic C2C12 cells. Furthermore, addition of LRR2-3 to osteoblastic MC3T3-E1 cells accelerated in vitro mineralization without compromising the quality of the mineral and matrix. These data indicate that LRR2-3 is, at least in part, responsible for BGN’s ability to enhance BMP-2 osteogenic function, and it could be useful for bone tissue regeneration.Prapaporn JongwattanapisanMasahiko TerajimaPatricia A. MiguezWilliam QueridoHideaki NagaokaNoriko SumidaElizabeth Grace GuryshKristy M. AinslieNancy PleshkoLalith PereraMitsuo YamauchiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018) |
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Medicine R Science Q |
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Medicine R Science Q Prapaporn Jongwattanapisan Masahiko Terajima Patricia A. Miguez William Querido Hideaki Nagaoka Noriko Sumida Elizabeth Grace Gurysh Kristy M. Ainslie Nancy Pleshko Lalith Perera Mitsuo Yamauchi Identification of the effector domain of biglycan that facilitates BMP-2 osteogenic function |
| description |
Abstract We have reported that recombinant biglycan (BGN) core protein accelerates bone formation in vivo by enhancing bone morphogenetic protein (BMP)-2 function. The purpose of the present study was to identify the specific domain (“effector”) within the BGN core protein that facilitates BMP-2 osteogenic function. Thus, we generated various recombinant and synthetic peptides corresponding to several domains of BGN, and tested their effects on BMP-2 functions in vitro. The results demonstrated that the leucine-rich repeats 2–3 domain (LRR2-3) of BGN significantly enhanced the BMP-2 induced Smad1/5/9 phosphorylation, osteogenic gene expression, and alkaline phosphatase activity in myogenic C2C12 cells. Furthermore, addition of LRR2-3 to osteoblastic MC3T3-E1 cells accelerated in vitro mineralization without compromising the quality of the mineral and matrix. These data indicate that LRR2-3 is, at least in part, responsible for BGN’s ability to enhance BMP-2 osteogenic function, and it could be useful for bone tissue regeneration. |
| format |
article |
| author |
Prapaporn Jongwattanapisan Masahiko Terajima Patricia A. Miguez William Querido Hideaki Nagaoka Noriko Sumida Elizabeth Grace Gurysh Kristy M. Ainslie Nancy Pleshko Lalith Perera Mitsuo Yamauchi |
| author_facet |
Prapaporn Jongwattanapisan Masahiko Terajima Patricia A. Miguez William Querido Hideaki Nagaoka Noriko Sumida Elizabeth Grace Gurysh Kristy M. Ainslie Nancy Pleshko Lalith Perera Mitsuo Yamauchi |
| author_sort |
Prapaporn Jongwattanapisan |
| title |
Identification of the effector domain of biglycan that facilitates BMP-2 osteogenic function |
| title_short |
Identification of the effector domain of biglycan that facilitates BMP-2 osteogenic function |
| title_full |
Identification of the effector domain of biglycan that facilitates BMP-2 osteogenic function |
| title_fullStr |
Identification of the effector domain of biglycan that facilitates BMP-2 osteogenic function |
| title_full_unstemmed |
Identification of the effector domain of biglycan that facilitates BMP-2 osteogenic function |
| title_sort |
identification of the effector domain of biglycan that facilitates bmp-2 osteogenic function |
| publisher |
Nature Portfolio |
| publishDate |
2018 |
| url |
https://doaj.org/article/86b12ac6f51546d392392532a8bb8bf5 |
| work_keys_str_mv |
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| _version_ |
1718394105177833472 |