To Discover the Efficient and Novel Drug Targets in Human Cancers Using CRISPR/Cas Screening and Databases

CRISPR/Cas has emerged as an excelle nt gene-editing technology and is used worldwide for research. The CRISPR library is an ideal tool for identifying essential genes and synthetic lethality targeted for cancer therapies in human cancers. Synthetic lethality is defined as multiple genetic abnormali...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Iichiroh Onishi, Kouhei Yamamoto, Yuko Kinowaki, Masanobu Kitagawa, Morito Kurata
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	CRISPR screening synthetic lethality database Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/875a5219042e49beb189ce78fc532fab
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:875a5219042e49beb189ce78fc532fab
record_format	dspace
spelling	oai:doaj.org-article:875a5219042e49beb189ce78fc532fab2021-11-25T17:55:24ZTo Discover the Efficient and Novel Drug Targets in Human Cancers Using CRISPR/Cas Screening and Databases10.3390/ijms2222123221422-00671661-6596https://doaj.org/article/875a5219042e49beb189ce78fc532fab2021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12322https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067CRISPR/Cas has emerged as an excelle nt gene-editing technology and is used worldwide for research. The CRISPR library is an ideal tool for identifying essential genes and synthetic lethality targeted for cancer therapies in human cancers. Synthetic lethality is defined as multiple genetic abnormalities that, when present individually, do not affect function or survival, but when present together, are lethal. Recently, many CRISPR libraries are available, and the latest libraries are more accurate and can be applied to few cells. However, it is easier to efficiently search for cancer targets with their own screenings by effectively using databases of CRISPR screenings, such as Depmap portal, PICKLES (Pooled In-Vitro CRISPR Knockout Library Essentiality Screens), iCSDB, Project Score database, and CRISP-view. This review will suggest recent optimal CRISPR libraries and effective databases for Novel Approaches in the Discovery and Design of Targeted Therapies.Iichiroh OnishiKouhei YamamotoYuko KinowakiMasanobu KitagawaMorito KurataMDPI AGarticleCRISPR screeningsynthetic lethalitydatabaseBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12322, p 12322 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	CRISPR screening synthetic lethality database Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	CRISPR screening synthetic lethality database Biology (General) QH301-705.5 Chemistry QD1-999 Iichiroh Onishi Kouhei Yamamoto Yuko Kinowaki Masanobu Kitagawa Morito Kurata To Discover the Efficient and Novel Drug Targets in Human Cancers Using CRISPR/Cas Screening and Databases
description	CRISPR/Cas has emerged as an excelle nt gene-editing technology and is used worldwide for research. The CRISPR library is an ideal tool for identifying essential genes and synthetic lethality targeted for cancer therapies in human cancers. Synthetic lethality is defined as multiple genetic abnormalities that, when present individually, do not affect function or survival, but when present together, are lethal. Recently, many CRISPR libraries are available, and the latest libraries are more accurate and can be applied to few cells. However, it is easier to efficiently search for cancer targets with their own screenings by effectively using databases of CRISPR screenings, such as Depmap portal, PICKLES (Pooled In-Vitro CRISPR Knockout Library Essentiality Screens), iCSDB, Project Score database, and CRISP-view. This review will suggest recent optimal CRISPR libraries and effective databases for Novel Approaches in the Discovery and Design of Targeted Therapies.
format	article
author	Iichiroh Onishi Kouhei Yamamoto Yuko Kinowaki Masanobu Kitagawa Morito Kurata
author_facet	Iichiroh Onishi Kouhei Yamamoto Yuko Kinowaki Masanobu Kitagawa Morito Kurata
author_sort	Iichiroh Onishi
title	To Discover the Efficient and Novel Drug Targets in Human Cancers Using CRISPR/Cas Screening and Databases
title_short	To Discover the Efficient and Novel Drug Targets in Human Cancers Using CRISPR/Cas Screening and Databases
title_full	To Discover the Efficient and Novel Drug Targets in Human Cancers Using CRISPR/Cas Screening and Databases
title_fullStr	To Discover the Efficient and Novel Drug Targets in Human Cancers Using CRISPR/Cas Screening and Databases
title_full_unstemmed	To Discover the Efficient and Novel Drug Targets in Human Cancers Using CRISPR/Cas Screening and Databases
title_sort	to discover the efficient and novel drug targets in human cancers using crispr/cas screening and databases
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/875a5219042e49beb189ce78fc532fab
work_keys_str_mv	AT iichirohonishi todiscovertheefficientandnoveldrugtargetsinhumancancersusingcrisprcasscreeninganddatabases AT kouheiyamamoto todiscovertheefficientandnoveldrugtargetsinhumancancersusingcrisprcasscreeninganddatabases AT yukokinowaki todiscovertheefficientandnoveldrugtargetsinhumancancersusingcrisprcasscreeninganddatabases AT masanobukitagawa todiscovertheefficientandnoveldrugtargetsinhumancancersusingcrisprcasscreeninganddatabases AT moritokurata todiscovertheefficientandnoveldrugtargetsinhumancancersusingcrisprcasscreeninganddatabases
_version_	1718411872904937472

To Discover the Efficient and Novel Drug Targets in Human Cancers Using CRISPR/Cas Screening and Databases

Ejemplares similares