A Universal Next-Generation Sequencing Protocol To Generate Noninfectious Barcoded cDNA Libraries from High-Containment RNA Viruses

A Universal Next-Generation Sequencing Protocol To Generate Noninfectious Barcoded cDNA Libraries from High-Containment RNA Viruses

ABSTRACT Several biosafety level 3 and/or 4 (BSL-3/4) pathogens are high-consequence, single-stranded RNA viruses, and their genomes, when introduced into permissive cells, are infectious. Moreover, many of these viruses are select agents (SAs), and their genomes are also considered SAs. For this re...

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Autores principales: Lindsey A. Moser, Lisbeth Ramirez-Carvajal, Vinita Puri, Steven J. Pauszek, Krystal Matthews, Kari A. Dilley, Clancy Mullan, Jennifer McGraw, Michael Khayat, Karen Beeri, Anthony Yee, Vivien Dugan, Mark T. Heise, Matthew B. Frieman, Luis L. Rodriguez, Kristen A. Bernard, David E. Wentworth, Timothy B. Stockwell, Reed S. Shabman
Formato: article
Lenguaje:EN
Publicado: American Society for Microbiology 2016
Materias:
next-generation sequencing
West Nile virus
alphavirus
coronavirus
flavivirus
foot-and-mouth disease virus
Microbiology
QR1-502
Acceso en línea:https://doaj.org/article/87969258f0004a58b311c0229c792bbc
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https://doaj.org/article/87969258f0004a58b311c0229c792bbc

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