TCR microclusters form spatially segregated domains and sequentially assemble in calcium-dependent kinetic steps

Engagement of T cell receptors (TCRs) induces the formation of microclusters that mediate the downstream signalling events. Here the authors show, using high resolution TIRF-SIM and live cell imaging, that ZAP70 and LAT are recruited to TCR with distinct kinetics, with the delayed ZAP70-TCR associat...

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Autores principales: Jason Yi, Lakshmi Balagopalan, Tiffany Nguyen, Katherine M. McIntire, Lawrence E. Samelson
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2019
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Acceso en línea:https://doaj.org/article/886499537f5d4d8a84ce8c1d223bc7f0
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spelling oai:doaj.org-article:886499537f5d4d8a84ce8c1d223bc7f02021-12-02T15:35:05ZTCR microclusters form spatially segregated domains and sequentially assemble in calcium-dependent kinetic steps10.1038/s41467-018-08064-22041-1723https://doaj.org/article/886499537f5d4d8a84ce8c1d223bc7f02019-01-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-08064-2https://doaj.org/toc/2041-1723Engagement of T cell receptors (TCRs) induces the formation of microclusters that mediate the downstream signalling events. Here the authors show, using high resolution TIRF-SIM and live cell imaging, that ZAP70 and LAT are recruited to TCR with distinct kinetics, with the delayed ZAP70-TCR association modulated by TCR-induced calcium flux.Jason YiLakshmi BalagopalanTiffany NguyenKatherine M. McIntireLawrence E. SamelsonNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-13 (2019)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Jason Yi
Lakshmi Balagopalan
Tiffany Nguyen
Katherine M. McIntire
Lawrence E. Samelson
TCR microclusters form spatially segregated domains and sequentially assemble in calcium-dependent kinetic steps
description Engagement of T cell receptors (TCRs) induces the formation of microclusters that mediate the downstream signalling events. Here the authors show, using high resolution TIRF-SIM and live cell imaging, that ZAP70 and LAT are recruited to TCR with distinct kinetics, with the delayed ZAP70-TCR association modulated by TCR-induced calcium flux.
format article
author Jason Yi
Lakshmi Balagopalan
Tiffany Nguyen
Katherine M. McIntire
Lawrence E. Samelson
author_facet Jason Yi
Lakshmi Balagopalan
Tiffany Nguyen
Katherine M. McIntire
Lawrence E. Samelson
author_sort Jason Yi
title TCR microclusters form spatially segregated domains and sequentially assemble in calcium-dependent kinetic steps
title_short TCR microclusters form spatially segregated domains and sequentially assemble in calcium-dependent kinetic steps
title_full TCR microclusters form spatially segregated domains and sequentially assemble in calcium-dependent kinetic steps
title_fullStr TCR microclusters form spatially segregated domains and sequentially assemble in calcium-dependent kinetic steps
title_full_unstemmed TCR microclusters form spatially segregated domains and sequentially assemble in calcium-dependent kinetic steps
title_sort tcr microclusters form spatially segregated domains and sequentially assemble in calcium-dependent kinetic steps
publisher Nature Portfolio
publishDate 2019
url https://doaj.org/article/886499537f5d4d8a84ce8c1d223bc7f0
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AT lakshmibalagopalan tcrmicroclustersformspatiallysegregateddomainsandsequentiallyassembleincalciumdependentkineticsteps
AT tiffanynguyen tcrmicroclustersformspatiallysegregateddomainsandsequentiallyassembleincalciumdependentkineticsteps
AT katherinemmcintire tcrmicroclustersformspatiallysegregateddomainsandsequentiallyassembleincalciumdependentkineticsteps
AT lawrenceesamelson tcrmicroclustersformspatiallysegregateddomainsandsequentiallyassembleincalciumdependentkineticsteps
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