TCR microclusters form spatially segregated domains and sequentially assemble in calcium-dependent kinetic steps
Engagement of T cell receptors (TCRs) induces the formation of microclusters that mediate the downstream signalling events. Here the authors show, using high resolution TIRF-SIM and live cell imaging, that ZAP70 and LAT are recruited to TCR with distinct kinetics, with the delayed ZAP70-TCR associat...
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Nature Portfolio
2019
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oai:doaj.org-article:886499537f5d4d8a84ce8c1d223bc7f02021-12-02T15:35:05ZTCR microclusters form spatially segregated domains and sequentially assemble in calcium-dependent kinetic steps10.1038/s41467-018-08064-22041-1723https://doaj.org/article/886499537f5d4d8a84ce8c1d223bc7f02019-01-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-08064-2https://doaj.org/toc/2041-1723Engagement of T cell receptors (TCRs) induces the formation of microclusters that mediate the downstream signalling events. Here the authors show, using high resolution TIRF-SIM and live cell imaging, that ZAP70 and LAT are recruited to TCR with distinct kinetics, with the delayed ZAP70-TCR association modulated by TCR-induced calcium flux.Jason YiLakshmi BalagopalanTiffany NguyenKatherine M. McIntireLawrence E. SamelsonNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-13 (2019) |
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Science Q Jason Yi Lakshmi Balagopalan Tiffany Nguyen Katherine M. McIntire Lawrence E. Samelson TCR microclusters form spatially segregated domains and sequentially assemble in calcium-dependent kinetic steps |
description |
Engagement of T cell receptors (TCRs) induces the formation of microclusters that mediate the downstream signalling events. Here the authors show, using high resolution TIRF-SIM and live cell imaging, that ZAP70 and LAT are recruited to TCR with distinct kinetics, with the delayed ZAP70-TCR association modulated by TCR-induced calcium flux. |
format |
article |
author |
Jason Yi Lakshmi Balagopalan Tiffany Nguyen Katherine M. McIntire Lawrence E. Samelson |
author_facet |
Jason Yi Lakshmi Balagopalan Tiffany Nguyen Katherine M. McIntire Lawrence E. Samelson |
author_sort |
Jason Yi |
title |
TCR microclusters form spatially segregated domains and sequentially assemble in calcium-dependent kinetic steps |
title_short |
TCR microclusters form spatially segregated domains and sequentially assemble in calcium-dependent kinetic steps |
title_full |
TCR microclusters form spatially segregated domains and sequentially assemble in calcium-dependent kinetic steps |
title_fullStr |
TCR microclusters form spatially segregated domains and sequentially assemble in calcium-dependent kinetic steps |
title_full_unstemmed |
TCR microclusters form spatially segregated domains and sequentially assemble in calcium-dependent kinetic steps |
title_sort |
tcr microclusters form spatially segregated domains and sequentially assemble in calcium-dependent kinetic steps |
publisher |
Nature Portfolio |
publishDate |
2019 |
url |
https://doaj.org/article/886499537f5d4d8a84ce8c1d223bc7f0 |
work_keys_str_mv |
AT jasonyi tcrmicroclustersformspatiallysegregateddomainsandsequentiallyassembleincalciumdependentkineticsteps AT lakshmibalagopalan tcrmicroclustersformspatiallysegregateddomainsandsequentiallyassembleincalciumdependentkineticsteps AT tiffanynguyen tcrmicroclustersformspatiallysegregateddomainsandsequentiallyassembleincalciumdependentkineticsteps AT katherinemmcintire tcrmicroclustersformspatiallysegregateddomainsandsequentiallyassembleincalciumdependentkineticsteps AT lawrenceesamelson tcrmicroclustersformspatiallysegregateddomainsandsequentiallyassembleincalciumdependentkineticsteps |
_version_ |
1718386667937595392 |