TCR microclusters form spatially segregated domains and sequentially assemble in calcium-dependent kinetic steps

Engagement of T cell receptors (TCRs) induces the formation of microclusters that mediate the downstream signalling events. Here the authors show, using high resolution TIRF-SIM and live cell imaging, that ZAP70 and LAT are recruited to TCR with distinct kinetics, with the delayed ZAP70-TCR associat...

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Main Authors:	Jason Yi, Lakshmi Balagopalan, Tiffany Nguyen, Katherine M. McIntire, Lawrence E. Samelson
Format:	article
Language:	EN
Published:	Nature Portfolio 2019
Subjects:	Science Q
Online Access:	https://doaj.org/article/886499537f5d4d8a84ce8c1d223bc7f0
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id	oai:doaj.org-article:886499537f5d4d8a84ce8c1d223bc7f0
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spelling	oai:doaj.org-article:886499537f5d4d8a84ce8c1d223bc7f02021-12-02T15:35:05ZTCR microclusters form spatially segregated domains and sequentially assemble in calcium-dependent kinetic steps10.1038/s41467-018-08064-22041-1723https://doaj.org/article/886499537f5d4d8a84ce8c1d223bc7f02019-01-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-08064-2https://doaj.org/toc/2041-1723Engagement of T cell receptors (TCRs) induces the formation of microclusters that mediate the downstream signalling events. Here the authors show, using high resolution TIRF-SIM and live cell imaging, that ZAP70 and LAT are recruited to TCR with distinct kinetics, with the delayed ZAP70-TCR association modulated by TCR-induced calcium flux.Jason YiLakshmi BalagopalanTiffany NguyenKatherine M. McIntireLawrence E. SamelsonNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-13 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Jason Yi Lakshmi Balagopalan Tiffany Nguyen Katherine M. McIntire Lawrence E. Samelson TCR microclusters form spatially segregated domains and sequentially assemble in calcium-dependent kinetic steps
description	Engagement of T cell receptors (TCRs) induces the formation of microclusters that mediate the downstream signalling events. Here the authors show, using high resolution TIRF-SIM and live cell imaging, that ZAP70 and LAT are recruited to TCR with distinct kinetics, with the delayed ZAP70-TCR association modulated by TCR-induced calcium flux.
format	article
author	Jason Yi Lakshmi Balagopalan Tiffany Nguyen Katherine M. McIntire Lawrence E. Samelson
author_facet	Jason Yi Lakshmi Balagopalan Tiffany Nguyen Katherine M. McIntire Lawrence E. Samelson
author_sort	Jason Yi
title	TCR microclusters form spatially segregated domains and sequentially assemble in calcium-dependent kinetic steps
title_short	TCR microclusters form spatially segregated domains and sequentially assemble in calcium-dependent kinetic steps
title_full	TCR microclusters form spatially segregated domains and sequentially assemble in calcium-dependent kinetic steps
title_fullStr	TCR microclusters form spatially segregated domains and sequentially assemble in calcium-dependent kinetic steps
title_full_unstemmed	TCR microclusters form spatially segregated domains and sequentially assemble in calcium-dependent kinetic steps
title_sort	tcr microclusters form spatially segregated domains and sequentially assemble in calcium-dependent kinetic steps
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/886499537f5d4d8a84ce8c1d223bc7f0
work_keys_str_mv	AT jasonyi tcrmicroclustersformspatiallysegregateddomainsandsequentiallyassembleincalciumdependentkineticsteps AT lakshmibalagopalan tcrmicroclustersformspatiallysegregateddomainsandsequentiallyassembleincalciumdependentkineticsteps AT tiffanynguyen tcrmicroclustersformspatiallysegregateddomainsandsequentiallyassembleincalciumdependentkineticsteps AT katherinemmcintire tcrmicroclustersformspatiallysegregateddomainsandsequentiallyassembleincalciumdependentkineticsteps AT lawrenceesamelson tcrmicroclustersformspatiallysegregateddomainsandsequentiallyassembleincalciumdependentkineticsteps
_version_	1718386667937595392

TCR microclusters form spatially segregated domains and sequentially assemble in calcium-dependent kinetic steps

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