Rolling circle transcription of ribozymes from promoterless single-stranded DNA nanocircles in mammalian cells [Ribozimlerin, promotorsuz tek-iplikli DNA çemberlerinin rolling circle transkripsiyonu ile memeli hücrelerinde üretimi]

Self-processing hairpin ribozymes have been synthesized from promoterless singlestranded DNA circles (73 nt) within mammalian cells. Following lipid-mediated transient transfection, DNA circles were efficiently internalized by mouse L cells (OST7–1) that stably express T7 RNA polymerase confining...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autor principal:	Attila A. Seyhan
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2006
Materias:	T7 RNA polymerase RNA polymerase II rTth DNA polymerase rolling circle transcription multimeric RNAs ribozyme catalysis circular DNA Biochemistry QD415-436
Acceso en línea:	https://doaj.org/article/887677e0d0894a6b980c8dd7497b3fb1
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:887677e0d0894a6b980c8dd7497b3fb1
record_format	dspace
spelling	oai:doaj.org-article:887677e0d0894a6b980c8dd7497b3fb12021-12-02T13:24:30ZRolling circle transcription of ribozymes from promoterless single-stranded DNA nanocircles in mammalian cells [Ribozimlerin, promotorsuz tek-iplikli DNA çemberlerinin rolling circle transkripsiyonu ile memeli hücrelerinde üretimi]0250-46851303-829Xhttps://doaj.org/article/887677e0d0894a6b980c8dd7497b3fb12006-03-01T00:00:00Zhttp://www.turkjbiochem.com/2006/002_012.pdfhttps://doaj.org/toc/0250-4685https://doaj.org/toc/1303-829XSelf-processing hairpin ribozymes have been synthesized from promoterless singlestranded DNA circles (73 nt) within mammalian cells. Following lipid-mediated transient transfection, DNA circles were efficiently internalized by mouse L cells (OST7–1) that stably express T7 RNA polymerase confining it to the cytoplasm. Cellular uptake of circular DNA templates and intracellular accumulation of ribozyme RNA transcripts from these DNA circles were progressive, both peaking at 24 h after transfection. Intracellular transcription generated RNA concatemers accumulating to a level of ~100 copies per cell. Transcription appears to be independent of specific promoter sequences but depends on T7 RNA polymerase. The data presented here may support the hypothesis that single stranded bubble regions within duplex DNA can serve as de novo initiation sites for RNA transcription not only in vitro but also in the cytoplasm of mammalian cells. These results may provide a model for the rolling circle transcription of small circular nucleic acids in mammalian cells.Attila A. SeyhanDe GruyterarticleT7 RNA polymeraseRNA polymerase IIrTth DNA polymeraserolling circle transcriptionmultimeric RNAsribozyme catalysiscircular DNABiochemistryQD415-436ENTürk Biyokimya Dergisi, Vol 31, Iss 1, Pp 2-12 (2006)
institution	DOAJ
collection	DOAJ
language	EN
topic	T7 RNA polymerase RNA polymerase II rTth DNA polymerase rolling circle transcription multimeric RNAs ribozyme catalysis circular DNA Biochemistry QD415-436
spellingShingle	T7 RNA polymerase RNA polymerase II rTth DNA polymerase rolling circle transcription multimeric RNAs ribozyme catalysis circular DNA Biochemistry QD415-436 Attila A. Seyhan Rolling circle transcription of ribozymes from promoterless single-stranded DNA nanocircles in mammalian cells [Ribozimlerin, promotorsuz tek-iplikli DNA çemberlerinin rolling circle transkripsiyonu ile memeli hücrelerinde üretimi]
description	Self-processing hairpin ribozymes have been synthesized from promoterless singlestranded DNA circles (73 nt) within mammalian cells. Following lipid-mediated transient transfection, DNA circles were efficiently internalized by mouse L cells (OST7–1) that stably express T7 RNA polymerase confining it to the cytoplasm. Cellular uptake of circular DNA templates and intracellular accumulation of ribozyme RNA transcripts from these DNA circles were progressive, both peaking at 24 h after transfection. Intracellular transcription generated RNA concatemers accumulating to a level of ~100 copies per cell. Transcription appears to be independent of specific promoter sequences but depends on T7 RNA polymerase. The data presented here may support the hypothesis that single stranded bubble regions within duplex DNA can serve as de novo initiation sites for RNA transcription not only in vitro but also in the cytoplasm of mammalian cells. These results may provide a model for the rolling circle transcription of small circular nucleic acids in mammalian cells.
format	article
author	Attila A. Seyhan
author_facet	Attila A. Seyhan
author_sort	Attila A. Seyhan
title	Rolling circle transcription of ribozymes from promoterless single-stranded DNA nanocircles in mammalian cells [Ribozimlerin, promotorsuz tek-iplikli DNA çemberlerinin rolling circle transkripsiyonu ile memeli hücrelerinde üretimi]
title_short	Rolling circle transcription of ribozymes from promoterless single-stranded DNA nanocircles in mammalian cells [Ribozimlerin, promotorsuz tek-iplikli DNA çemberlerinin rolling circle transkripsiyonu ile memeli hücrelerinde üretimi]
title_full	Rolling circle transcription of ribozymes from promoterless single-stranded DNA nanocircles in mammalian cells [Ribozimlerin, promotorsuz tek-iplikli DNA çemberlerinin rolling circle transkripsiyonu ile memeli hücrelerinde üretimi]
title_fullStr	Rolling circle transcription of ribozymes from promoterless single-stranded DNA nanocircles in mammalian cells [Ribozimlerin, promotorsuz tek-iplikli DNA çemberlerinin rolling circle transkripsiyonu ile memeli hücrelerinde üretimi]
title_full_unstemmed	Rolling circle transcription of ribozymes from promoterless single-stranded DNA nanocircles in mammalian cells [Ribozimlerin, promotorsuz tek-iplikli DNA çemberlerinin rolling circle transkripsiyonu ile memeli hücrelerinde üretimi]
title_sort	rolling circle transcription of ribozymes from promoterless single-stranded dna nanocircles in mammalian cells [ribozimlerin, promotorsuz tek-iplikli dna çemberlerinin rolling circle transkripsiyonu ile memeli hücrelerinde üretimi]
publisher	De Gruyter
publishDate	2006
url	https://doaj.org/article/887677e0d0894a6b980c8dd7497b3fb1
work_keys_str_mv	AT attilaaseyhan rollingcircletranscriptionofribozymesfrompromoterlesssinglestrandeddnananocirclesinmammaliancellsribozimlerinpromotorsuztekipliklidnacemberlerininrollingcircletranskripsiyonuilememelihucrelerindeuretimi
_version_	1718393106465816576

Rolling circle transcription of ribozymes from promoterless single-stranded DNA nanocircles in mammalian cells [Ribozimlerin, promotorsuz tek-iplikli DNA çemberlerinin rolling circle transkripsiyonu ile memeli hücrelerinde üretimi]

Ejemplares similares