Flexible experimental designs for valid single-cell RNA-sequencing experiments allowing batch effects correction

It is not clear which designs, other than completely randomized ones, are valid for scRNA-seq experiments so that batch effects can be adjusted. Here the authors show that under flexible reference panel and chain-type designs, biological variability can also be separated from batch effects, at least...

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Autores principales:	Fangda Song, Ga Ming Angus Chan, Yingying Wei
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/889067254b4d4524ba0685f276a62942
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spelling	oai:doaj.org-article:889067254b4d4524ba0685f276a629422021-12-02T16:13:40ZFlexible experimental designs for valid single-cell RNA-sequencing experiments allowing batch effects correction10.1038/s41467-020-16905-22041-1723https://doaj.org/article/889067254b4d4524ba0685f276a629422020-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-16905-2https://doaj.org/toc/2041-1723It is not clear which designs, other than completely randomized ones, are valid for scRNA-seq experiments so that batch effects can be adjusted. Here the authors show that under flexible reference panel and chain-type designs, biological variability can also be separated from batch effects, at least by BUSseq.Fangda SongGa Ming Angus ChanYingying WeiNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-15 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Fangda Song Ga Ming Angus Chan Yingying Wei Flexible experimental designs for valid single-cell RNA-sequencing experiments allowing batch effects correction
description	It is not clear which designs, other than completely randomized ones, are valid for scRNA-seq experiments so that batch effects can be adjusted. Here the authors show that under flexible reference panel and chain-type designs, biological variability can also be separated from batch effects, at least by BUSseq.
format	article
author	Fangda Song Ga Ming Angus Chan Yingying Wei
author_facet	Fangda Song Ga Ming Angus Chan Yingying Wei
author_sort	Fangda Song
title	Flexible experimental designs for valid single-cell RNA-sequencing experiments allowing batch effects correction
title_short	Flexible experimental designs for valid single-cell RNA-sequencing experiments allowing batch effects correction
title_full	Flexible experimental designs for valid single-cell RNA-sequencing experiments allowing batch effects correction
title_fullStr	Flexible experimental designs for valid single-cell RNA-sequencing experiments allowing batch effects correction
title_full_unstemmed	Flexible experimental designs for valid single-cell RNA-sequencing experiments allowing batch effects correction
title_sort	flexible experimental designs for valid single-cell rna-sequencing experiments allowing batch effects correction
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/889067254b4d4524ba0685f276a62942
work_keys_str_mv	AT fangdasong flexibleexperimentaldesignsforvalidsinglecellrnasequencingexperimentsallowingbatcheffectscorrection AT gaminganguschan flexibleexperimentaldesignsforvalidsinglecellrnasequencingexperimentsallowingbatcheffectscorrection AT yingyingwei flexibleexperimentaldesignsforvalidsinglecellrnasequencingexperimentsallowingbatcheffectscorrection
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Flexible experimental designs for valid single-cell RNA-sequencing experiments allowing batch effects correction

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