CNOT6 regulates a novel pattern of mRNA deadenylation during oocyte meiotic maturation

Abstract In many cell types, the length of the poly(A) tail of an mRNA is closely linked to its fate - a long tail is associated with active translation, a short tail with silencing and degradation. During mammalian oocyte development, two contrasting patterns of polyadenylation have been identified...

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Autores principales:	Karl-Frédéric Vieux, Hugh J. Clarke
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/88be864bde564d92b1c953d226a7f749
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spelling	oai:doaj.org-article:88be864bde564d92b1c953d226a7f7492021-12-02T11:40:15ZCNOT6 regulates a novel pattern of mRNA deadenylation during oocyte meiotic maturation10.1038/s41598-018-25187-02045-2322https://doaj.org/article/88be864bde564d92b1c953d226a7f7492018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25187-0https://doaj.org/toc/2045-2322Abstract In many cell types, the length of the poly(A) tail of an mRNA is closely linked to its fate - a long tail is associated with active translation, a short tail with silencing and degradation. During mammalian oocyte development, two contrasting patterns of polyadenylation have been identified. Some mRNAs carry a long poly(A) tail during the growth stage and are actively translated, then become deadenylated and down-regulated during the subsequent stage, termed meiotic maturation. Other mRNAs carry a short tail poly(A) tail and are translationally repressed during growth, and their poly(A) tail lengthens and they become translationally activated during maturation. As well, a program of elimination of this ‘maternal’ mRNA is initiated during oocyte maturation. Here we describe a third pattern of polyadenylation: mRNAs are deadenylated in growing oocytes, become polyadenylated during early maturation and then deadenylated during late maturation. We show that the deadenylase, CNOT6, is present in cortical foci of oocytes and regulates deadenylation of these mRNAs, and that PUF-binding elements (PBEs) regulate deadenylation in mature oocytes. Unexpectedly, maintaining a long poly(A) tail neither enhances translation nor inhibits degradation of these mRNAs. Our findings implicate multiple machineries, more complex than previously thought, in regulating mRNA activity in oocytes.Karl-Frédéric VieuxHugh J. ClarkeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-14 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Karl-Frédéric Vieux Hugh J. Clarke CNOT6 regulates a novel pattern of mRNA deadenylation during oocyte meiotic maturation
description	Abstract In many cell types, the length of the poly(A) tail of an mRNA is closely linked to its fate - a long tail is associated with active translation, a short tail with silencing and degradation. During mammalian oocyte development, two contrasting patterns of polyadenylation have been identified. Some mRNAs carry a long poly(A) tail during the growth stage and are actively translated, then become deadenylated and down-regulated during the subsequent stage, termed meiotic maturation. Other mRNAs carry a short tail poly(A) tail and are translationally repressed during growth, and their poly(A) tail lengthens and they become translationally activated during maturation. As well, a program of elimination of this ‘maternal’ mRNA is initiated during oocyte maturation. Here we describe a third pattern of polyadenylation: mRNAs are deadenylated in growing oocytes, become polyadenylated during early maturation and then deadenylated during late maturation. We show that the deadenylase, CNOT6, is present in cortical foci of oocytes and regulates deadenylation of these mRNAs, and that PUF-binding elements (PBEs) regulate deadenylation in mature oocytes. Unexpectedly, maintaining a long poly(A) tail neither enhances translation nor inhibits degradation of these mRNAs. Our findings implicate multiple machineries, more complex than previously thought, in regulating mRNA activity in oocytes.
format	article
author	Karl-Frédéric Vieux Hugh J. Clarke
author_facet	Karl-Frédéric Vieux Hugh J. Clarke
author_sort	Karl-Frédéric Vieux
title	CNOT6 regulates a novel pattern of mRNA deadenylation during oocyte meiotic maturation
title_short	CNOT6 regulates a novel pattern of mRNA deadenylation during oocyte meiotic maturation
title_full	CNOT6 regulates a novel pattern of mRNA deadenylation during oocyte meiotic maturation
title_fullStr	CNOT6 regulates a novel pattern of mRNA deadenylation during oocyte meiotic maturation
title_full_unstemmed	CNOT6 regulates a novel pattern of mRNA deadenylation during oocyte meiotic maturation
title_sort	cnot6 regulates a novel pattern of mrna deadenylation during oocyte meiotic maturation
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/88be864bde564d92b1c953d226a7f749
work_keys_str_mv	AT karlfredericvieux cnot6regulatesanovelpatternofmrnadeadenylationduringoocytemeioticmaturation AT hughjclarke cnot6regulatesanovelpatternofmrnadeadenylationduringoocytemeioticmaturation
_version_	1718395657484500992

CNOT6 regulates a novel pattern of mRNA deadenylation during oocyte meiotic maturation

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