Genetic screens in isogenic mammalian cell lines without single cell cloning

Isogenic pairs of cell lines are powerful tools but time-consuming to generate. Here the authors conduct genome-wide genetic interactions screens of ‘anchor’ genes with SaCas9 and SpCas9.

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Autores principales: Peter C. DeWeirdt, Annabel K. Sangree, Ruth E. Hanna, Kendall R. Sanson, Mudra Hegde, Christine Strand, Nicole S. Persky, John G. Doench
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2020
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Acceso en línea:https://doaj.org/article/892a16ea5f874784a2584fee9329b79a
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