Genetic screens in isogenic mammalian cell lines without single cell cloning

Isogenic pairs of cell lines are powerful tools but time-consuming to generate. Here the authors conduct genome-wide genetic interactions screens of ‘anchor’ genes with SaCas9 and SpCas9.

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Autores principales:	Peter C. DeWeirdt, Annabel K. Sangree, Ruth E. Hanna, Kendall R. Sanson, Mudra Hegde, Christine Strand, Nicole S. Persky, John G. Doench
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/892a16ea5f874784a2584fee9329b79a
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spelling	oai:doaj.org-article:892a16ea5f874784a2584fee9329b79a2021-12-02T15:37:20ZGenetic screens in isogenic mammalian cell lines without single cell cloning10.1038/s41467-020-14620-62041-1723https://doaj.org/article/892a16ea5f874784a2584fee9329b79a2020-02-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-14620-6https://doaj.org/toc/2041-1723Isogenic pairs of cell lines are powerful tools but time-consuming to generate. Here the authors conduct genome-wide genetic interactions screens of ‘anchor’ genes with SaCas9 and SpCas9.Peter C. DeWeirdtAnnabel K. SangreeRuth E. HannaKendall R. SansonMudra HegdeChristine StrandNicole S. PerskyJohn G. DoenchNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-15 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Peter C. DeWeirdt Annabel K. Sangree Ruth E. Hanna Kendall R. Sanson Mudra Hegde Christine Strand Nicole S. Persky John G. Doench Genetic screens in isogenic mammalian cell lines without single cell cloning
description	Isogenic pairs of cell lines are powerful tools but time-consuming to generate. Here the authors conduct genome-wide genetic interactions screens of ‘anchor’ genes with SaCas9 and SpCas9.
format	article
author	Peter C. DeWeirdt Annabel K. Sangree Ruth E. Hanna Kendall R. Sanson Mudra Hegde Christine Strand Nicole S. Persky John G. Doench
author_facet	Peter C. DeWeirdt Annabel K. Sangree Ruth E. Hanna Kendall R. Sanson Mudra Hegde Christine Strand Nicole S. Persky John G. Doench
author_sort	Peter C. DeWeirdt
title	Genetic screens in isogenic mammalian cell lines without single cell cloning
title_short	Genetic screens in isogenic mammalian cell lines without single cell cloning
title_full	Genetic screens in isogenic mammalian cell lines without single cell cloning
title_fullStr	Genetic screens in isogenic mammalian cell lines without single cell cloning
title_full_unstemmed	Genetic screens in isogenic mammalian cell lines without single cell cloning
title_sort	genetic screens in isogenic mammalian cell lines without single cell cloning
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/892a16ea5f874784a2584fee9329b79a
work_keys_str_mv	AT petercdeweirdt geneticscreensinisogenicmammaliancelllineswithoutsinglecellcloning AT annabelksangree geneticscreensinisogenicmammaliancelllineswithoutsinglecellcloning AT ruthehanna geneticscreensinisogenicmammaliancelllineswithoutsinglecellcloning AT kendallrsanson geneticscreensinisogenicmammaliancelllineswithoutsinglecellcloning AT mudrahegde geneticscreensinisogenicmammaliancelllineswithoutsinglecellcloning AT christinestrand geneticscreensinisogenicmammaliancelllineswithoutsinglecellcloning AT nicolespersky geneticscreensinisogenicmammaliancelllineswithoutsinglecellcloning AT johngdoench geneticscreensinisogenicmammaliancelllineswithoutsinglecellcloning
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Genetic screens in isogenic mammalian cell lines without single cell cloning

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