Genetic screens in isogenic mammalian cell lines without single cell cloning

Genetic screens in isogenic mammalian cell lines without single cell cloning

Isogenic pairs of cell lines are powerful tools but time-consuming to generate. Here the authors conduct genome-wide genetic interactions screens of ‘anchor’ genes with SaCas9 and SpCas9.

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Detalles Bibliográficos
Autores principales: Peter C. DeWeirdt, Annabel K. Sangree, Ruth E. Hanna, Kendall R. Sanson, Mudra Hegde, Christine Strand, Nicole S. Persky, John G. Doench
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2020
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Science
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Acceso en línea:https://doaj.org/article/892a16ea5f874784a2584fee9329b79a
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