A dual prokaryotic (E. coli) expression system (pdMAX).

In this study, we introduced an efficient subcloning and expression system with two inducible prokaryotic expression promoters, arabinose and lac, in a single plasmid in Escherichia coli. The arabinose promoter unit allows for the expression of a FLAG-tagged protein, while the isopropyl-β-D-thiogala...

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Autores principales: Manabu Murakami, Agnieszka M Murakami, Shirou Itagaki
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2021
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Acceso en línea:https://doaj.org/article/898cb8e561ae4d2ca8da9687fe58dcca
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