Mechanical slowing-down of cytoplasmic diffusion allows in vivo counting of proteins in individual cells

Several proteins are expressed at too low abundance in the Escherichia coli (E. coli) proteome to be detected by standard methods. Here, the authors create a microfluidics-based platform enabling single-molecule counting of low-abundance proteins by mechanically slowing-down their diffusion in live...

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Auteurs principaux: Burak Okumus, Dirk Landgraf, Ghee Chuan Lai, Somenath Bakshi, Juan Carlos Arias-Castro, Sadik Yildiz, Dann Huh, Raul Fernandez-Lopez, Celeste N. Peterson, Erdal Toprak, Meriem El Karoui, Johan Paulsson
Format: article
Langue:EN
Publié: Nature Portfolio 2016
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Accès en ligne:https://doaj.org/article/8a1232042ef1474fa593b28506b6483e
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