Mechanical slowing-down of cytoplasmic diffusion allows in vivo counting of proteins in individual cells

Several proteins are expressed at too low abundance in the Escherichia coli (E. coli) proteome to be detected by standard methods. Here, the authors create a microfluidics-based platform enabling single-molecule counting of low-abundance proteins by mechanically slowing-down their diffusion in live...

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Autores principales:	Burak Okumus, Dirk Landgraf, Ghee Chuan Lai, Somenath Bakshi, Juan Carlos Arias-Castro, Sadik Yildiz, Dann Huh, Raul Fernandez-Lopez, Celeste N. Peterson, Erdal Toprak, Meriem El Karoui, Johan Paulsson
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2016
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/8a1232042ef1474fa593b28506b6483e
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spelling	oai:doaj.org-article:8a1232042ef1474fa593b28506b6483e2021-12-02T14:38:55ZMechanical slowing-down of cytoplasmic diffusion allows in vivo counting of proteins in individual cells10.1038/ncomms116412041-1723https://doaj.org/article/8a1232042ef1474fa593b28506b6483e2016-05-01T00:00:00Zhttps://doi.org/10.1038/ncomms11641https://doaj.org/toc/2041-1723Several proteins are expressed at too low abundance in the Escherichia coli (E. coli) proteome to be detected by standard methods. Here, the authors create a microfluidics-based platform enabling single-molecule counting of low-abundance proteins by mechanically slowing-down their diffusion in live E. coli.Burak OkumusDirk LandgrafGhee Chuan LaiSomenath BakshiJuan Carlos Arias-CastroSadik YildizDann HuhRaul Fernandez-LopezCeleste N. PetersonErdal ToprakMeriem El KarouiJohan PaulssonNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-11 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Burak Okumus Dirk Landgraf Ghee Chuan Lai Somenath Bakshi Juan Carlos Arias-Castro Sadik Yildiz Dann Huh Raul Fernandez-Lopez Celeste N. Peterson Erdal Toprak Meriem El Karoui Johan Paulsson Mechanical slowing-down of cytoplasmic diffusion allows in vivo counting of proteins in individual cells
description	Several proteins are expressed at too low abundance in the Escherichia coli (E. coli) proteome to be detected by standard methods. Here, the authors create a microfluidics-based platform enabling single-molecule counting of low-abundance proteins by mechanically slowing-down their diffusion in live E. coli.
format	article
author	Burak Okumus Dirk Landgraf Ghee Chuan Lai Somenath Bakshi Juan Carlos Arias-Castro Sadik Yildiz Dann Huh Raul Fernandez-Lopez Celeste N. Peterson Erdal Toprak Meriem El Karoui Johan Paulsson
author_facet	Burak Okumus Dirk Landgraf Ghee Chuan Lai Somenath Bakshi Juan Carlos Arias-Castro Sadik Yildiz Dann Huh Raul Fernandez-Lopez Celeste N. Peterson Erdal Toprak Meriem El Karoui Johan Paulsson
author_sort	Burak Okumus
title	Mechanical slowing-down of cytoplasmic diffusion allows in vivo counting of proteins in individual cells
title_short	Mechanical slowing-down of cytoplasmic diffusion allows in vivo counting of proteins in individual cells
title_full	Mechanical slowing-down of cytoplasmic diffusion allows in vivo counting of proteins in individual cells
title_fullStr	Mechanical slowing-down of cytoplasmic diffusion allows in vivo counting of proteins in individual cells
title_full_unstemmed	Mechanical slowing-down of cytoplasmic diffusion allows in vivo counting of proteins in individual cells
title_sort	mechanical slowing-down of cytoplasmic diffusion allows in vivo counting of proteins in individual cells
publisher	Nature Portfolio
publishDate	2016
url	https://doaj.org/article/8a1232042ef1474fa593b28506b6483e
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Mechanical slowing-down of cytoplasmic diffusion allows in vivo counting of proteins in individual cells

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