Hydrogen bonds in polycation improve the gene delivery efficiency in the serum-containing environment

Cationic polymers with high charge density could effectively condense the DNA and achieve gene transfection; however, it often brings non-negligible cytotoxicity. Notably, the high charge density gene vector fails in the serum environment, limiting further application in vivo. In this paper, an effi...

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Autores principales:	Smaher M. Elbayomi, Xuan Nie, Ye-Zi You, Tamer M. Tamer
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Gene delivery Non-viral vector Hydrogen bond Serum tolerance Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/8a3a76071f8f45f3b4780d0372349781
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spelling	oai:doaj.org-article:8a3a76071f8f45f3b4780d03723497812021-11-18T04:45:54ZHydrogen bonds in polycation improve the gene delivery efficiency in the serum-containing environment1319-610310.1016/j.jscs.2021.101361https://doaj.org/article/8a3a76071f8f45f3b4780d03723497812021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1319610321001666https://doaj.org/toc/1319-6103Cationic polymers with high charge density could effectively condense the DNA and achieve gene transfection; however, it often brings non-negligible cytotoxicity. Notably, the high charge density gene vector fails in the serum environment, limiting further application in vivo. In this paper, an efficient and reliable non-viral gene vector of poly (amidoamine) (PAA) was designed by introducing diacryolyl-2,6-diaminopyridine (DADAP) onto the PAA backbone through Michael-addition polymerization, which provides high transfection efficiency in a serum-containing environment. Diacryolyl-2,6-diaminopyridine and cationic parts provided multiple interactions between gene vectors and DNA, including hydrogen bond and electrostatic interactions. The introduction of hydrogen bonding can effectively reduce the charge density of polyplexes without reducing the DNA condensing ability, incorporating the diaminopyridine group and cationic part in PAA chains successfully consolidated cellular uptake, endosome destabilization, and transfection efficiency for the PAA/DNA complexes with low cytotoxicity. The constructed vector with multiple interactions presented 6 times higher transfection efficiency in serum-free and 9 times in serum-containing environment than that of branched polyethyleneimine (PEI 25K) in 293T cells in vitro. Therefore, introducing the hydrogen band to form low charge density polyplexes with high transfection efficiency and low cytotoxicity has a great potential in gene delivery.Smaher M. ElbayomiXuan NieYe-Zi YouTamer M. TamerElsevierarticleGene deliveryNon-viral vectorHydrogen bondSerum toleranceChemistryQD1-999ENJournal of Saudi Chemical Society, Vol 25, Iss 12, Pp 101361- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Gene delivery Non-viral vector Hydrogen bond Serum tolerance Chemistry QD1-999
spellingShingle	Gene delivery Non-viral vector Hydrogen bond Serum tolerance Chemistry QD1-999 Smaher M. Elbayomi Xuan Nie Ye-Zi You Tamer M. Tamer Hydrogen bonds in polycation improve the gene delivery efficiency in the serum-containing environment
description	Cationic polymers with high charge density could effectively condense the DNA and achieve gene transfection; however, it often brings non-negligible cytotoxicity. Notably, the high charge density gene vector fails in the serum environment, limiting further application in vivo. In this paper, an efficient and reliable non-viral gene vector of poly (amidoamine) (PAA) was designed by introducing diacryolyl-2,6-diaminopyridine (DADAP) onto the PAA backbone through Michael-addition polymerization, which provides high transfection efficiency in a serum-containing environment. Diacryolyl-2,6-diaminopyridine and cationic parts provided multiple interactions between gene vectors and DNA, including hydrogen bond and electrostatic interactions. The introduction of hydrogen bonding can effectively reduce the charge density of polyplexes without reducing the DNA condensing ability, incorporating the diaminopyridine group and cationic part in PAA chains successfully consolidated cellular uptake, endosome destabilization, and transfection efficiency for the PAA/DNA complexes with low cytotoxicity. The constructed vector with multiple interactions presented 6 times higher transfection efficiency in serum-free and 9 times in serum-containing environment than that of branched polyethyleneimine (PEI 25K) in 293T cells in vitro. Therefore, introducing the hydrogen band to form low charge density polyplexes with high transfection efficiency and low cytotoxicity has a great potential in gene delivery.
format	article
author	Smaher M. Elbayomi Xuan Nie Ye-Zi You Tamer M. Tamer
author_facet	Smaher M. Elbayomi Xuan Nie Ye-Zi You Tamer M. Tamer
author_sort	Smaher M. Elbayomi
title	Hydrogen bonds in polycation improve the gene delivery efficiency in the serum-containing environment
title_short	Hydrogen bonds in polycation improve the gene delivery efficiency in the serum-containing environment
title_full	Hydrogen bonds in polycation improve the gene delivery efficiency in the serum-containing environment
title_fullStr	Hydrogen bonds in polycation improve the gene delivery efficiency in the serum-containing environment
title_full_unstemmed	Hydrogen bonds in polycation improve the gene delivery efficiency in the serum-containing environment
title_sort	hydrogen bonds in polycation improve the gene delivery efficiency in the serum-containing environment
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/8a3a76071f8f45f3b4780d0372349781
work_keys_str_mv	AT smahermelbayomi hydrogenbondsinpolycationimprovethegenedeliveryefficiencyintheserumcontainingenvironment AT xuannie hydrogenbondsinpolycationimprovethegenedeliveryefficiencyintheserumcontainingenvironment AT yeziyou hydrogenbondsinpolycationimprovethegenedeliveryefficiencyintheserumcontainingenvironment AT tamermtamer hydrogenbondsinpolycationimprovethegenedeliveryefficiencyintheserumcontainingenvironment
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Hydrogen bonds in polycation improve the gene delivery efficiency in the serum-containing environment

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