Single particle cryo-EM reconstruction of 52 kDa streptavidin at 3.2 Angstrom resolution

It remains a challenge to obtain high-resolution structures of molecules smaller than 200 kDa using single particle cryo-EM. Here, the authors apply the Cs-corrector-VPP coupled cryo-EM and solve structures of the 52 kDa streptavidin (SA) protein at near-atomic resolution.

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Auteurs principaux: Xiao Fan, Jia Wang, Xing Zhang, Zi Yang, Jin-Can Zhang, Lingyun Zhao, Hai-Lin Peng, Jianlin Lei, Hong-Wei Wang
Format: article
Langue:EN
Publié: Nature Portfolio 2019
Sujets:
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Accès en ligne:https://doaj.org/article/8a89421a2bf6405aa235a43e887d674f
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