Genotype-Dependent Immunogenicity of Dengue Virus Type 2 Asian I and Asian/American Genotypes in Common Marmoset (<i>Callithrix jacchus)</i>: Discrepancy in Neutralizing and Infection-Enhancing Antibody Levels between Genotypes

Owing to genotype-specific neutralizing antibodies, analyzing differences in the immunogenic variation among dengue virus (DENV) genotypes is central to effective vaccine development. Herein, we characterized the viral kinetics and antibody response induced by DENV type 2 Asian I (AI) and Asian/Amer...

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Autores principales:	Nor Azila Muhammad Azami, Meng Ling Moi, Yasushi Ami, Yuriko Suzaki, Satoshi Taniguchi, Shigeru Tajima, Masayuki Saijo, Tomohiko Takasaki, Ichiro Kurane, Chang-Kweng Lim
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	dengue common marmoset neutralizing antibody genotypes FcγR-expressing BHK cells infection-enhancing Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/8b045d0ea8134f1d9ae4d1c1dac49f35
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spelling	oai:doaj.org-article:8b045d0ea8134f1d9ae4d1c1dac49f352021-11-25T18:24:12ZGenotype-Dependent Immunogenicity of Dengue Virus Type 2 Asian I and Asian/American Genotypes in Common Marmoset (<i>Callithrix jacchus)</i>: Discrepancy in Neutralizing and Infection-Enhancing Antibody Levels between Genotypes10.3390/microorganisms91121962076-2607https://doaj.org/article/8b045d0ea8134f1d9ae4d1c1dac49f352021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2196https://doaj.org/toc/2076-2607Owing to genotype-specific neutralizing antibodies, analyzing differences in the immunogenic variation among dengue virus (DENV) genotypes is central to effective vaccine development. Herein, we characterized the viral kinetics and antibody response induced by DENV type 2 Asian I (AI) and Asian/American (AA) genotypes using marmosets (<i>Callithrix jacchus</i>) as models. Two groups of marmosets were inoculated with AI and AA genotypes, and serial plasma samples were collected. Viremia levels were determined using quantitative reverse transcription-PCR, plaque assays, and antigen enzyme-linked immunosorbent assay (ELISA). Anti-DENV immunoglobulin M and G antibodies, neutralizing antibody titer, and antibody-dependent enhancement (ADE) activity were determined using ELISA, plaque reduction neutralization test, and ADE assay, respectively. The AI genotype induced viremia for a longer duration, but the AA genotype induced higher levels of viremia. After four months, the neutralizing antibody titer induced by the AA genotype remained high, but that induced by the AI genotype waned. ADE activity toward Cosmopolitan genotypes was detected in marmosets inoculated with the AI genotype. These findings indicate discrepancies between heterologous genotypes that influence neutralizing antibodies and viremia in marmosets, a critical issue in vaccine development.Nor Azila Muhammad AzamiMeng Ling MoiYasushi AmiYuriko SuzakiSatoshi TaniguchiShigeru TajimaMasayuki SaijoTomohiko TakasakiIchiro KuraneChang-Kweng LimMDPI AGarticledenguecommon marmosetneutralizing antibodygenotypesFcγR-expressing BHK cellsinfection-enhancingBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2196, p 2196 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	dengue common marmoset neutralizing antibody genotypes FcγR-expressing BHK cells infection-enhancing Biology (General) QH301-705.5
spellingShingle	dengue common marmoset neutralizing antibody genotypes FcγR-expressing BHK cells infection-enhancing Biology (General) QH301-705.5 Nor Azila Muhammad Azami Meng Ling Moi Yasushi Ami Yuriko Suzaki Satoshi Taniguchi Shigeru Tajima Masayuki Saijo Tomohiko Takasaki Ichiro Kurane Chang-Kweng Lim Genotype-Dependent Immunogenicity of Dengue Virus Type 2 Asian I and Asian/American Genotypes in Common Marmoset (<i>Callithrix jacchus)</i>: Discrepancy in Neutralizing and Infection-Enhancing Antibody Levels between Genotypes
description	Owing to genotype-specific neutralizing antibodies, analyzing differences in the immunogenic variation among dengue virus (DENV) genotypes is central to effective vaccine development. Herein, we characterized the viral kinetics and antibody response induced by DENV type 2 Asian I (AI) and Asian/American (AA) genotypes using marmosets (<i>Callithrix jacchus</i>) as models. Two groups of marmosets were inoculated with AI and AA genotypes, and serial plasma samples were collected. Viremia levels were determined using quantitative reverse transcription-PCR, plaque assays, and antigen enzyme-linked immunosorbent assay (ELISA). Anti-DENV immunoglobulin M and G antibodies, neutralizing antibody titer, and antibody-dependent enhancement (ADE) activity were determined using ELISA, plaque reduction neutralization test, and ADE assay, respectively. The AI genotype induced viremia for a longer duration, but the AA genotype induced higher levels of viremia. After four months, the neutralizing antibody titer induced by the AA genotype remained high, but that induced by the AI genotype waned. ADE activity toward Cosmopolitan genotypes was detected in marmosets inoculated with the AI genotype. These findings indicate discrepancies between heterologous genotypes that influence neutralizing antibodies and viremia in marmosets, a critical issue in vaccine development.
format	article
author	Nor Azila Muhammad Azami Meng Ling Moi Yasushi Ami Yuriko Suzaki Satoshi Taniguchi Shigeru Tajima Masayuki Saijo Tomohiko Takasaki Ichiro Kurane Chang-Kweng Lim
author_facet	Nor Azila Muhammad Azami Meng Ling Moi Yasushi Ami Yuriko Suzaki Satoshi Taniguchi Shigeru Tajima Masayuki Saijo Tomohiko Takasaki Ichiro Kurane Chang-Kweng Lim
author_sort	Nor Azila Muhammad Azami
title	Genotype-Dependent Immunogenicity of Dengue Virus Type 2 Asian I and Asian/American Genotypes in Common Marmoset (<i>Callithrix jacchus)</i>: Discrepancy in Neutralizing and Infection-Enhancing Antibody Levels between Genotypes
title_short	Genotype-Dependent Immunogenicity of Dengue Virus Type 2 Asian I and Asian/American Genotypes in Common Marmoset (<i>Callithrix jacchus)</i>: Discrepancy in Neutralizing and Infection-Enhancing Antibody Levels between Genotypes
title_full	Genotype-Dependent Immunogenicity of Dengue Virus Type 2 Asian I and Asian/American Genotypes in Common Marmoset (<i>Callithrix jacchus)</i>: Discrepancy in Neutralizing and Infection-Enhancing Antibody Levels between Genotypes
title_fullStr	Genotype-Dependent Immunogenicity of Dengue Virus Type 2 Asian I and Asian/American Genotypes in Common Marmoset (<i>Callithrix jacchus)</i>: Discrepancy in Neutralizing and Infection-Enhancing Antibody Levels between Genotypes
title_full_unstemmed	Genotype-Dependent Immunogenicity of Dengue Virus Type 2 Asian I and Asian/American Genotypes in Common Marmoset (<i>Callithrix jacchus)</i>: Discrepancy in Neutralizing and Infection-Enhancing Antibody Levels between Genotypes
title_sort	genotype-dependent immunogenicity of dengue virus type 2 asian i and asian/american genotypes in common marmoset (<i>callithrix jacchus)</i>: discrepancy in neutralizing and infection-enhancing antibody levels between genotypes
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/8b045d0ea8134f1d9ae4d1c1dac49f35
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Genotype-Dependent Immunogenicity of Dengue Virus Type 2 Asian I and Asian/American Genotypes in Common Marmoset (<i>Callithrix jacchus)</i>: Discrepancy in Neutralizing and Infection-Enhancing Antibody Levels between Genotypes

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