A combined microphysiological-computational omics approach in dietary protein evaluation
Abstract Food security is under increased pressure due to the ever-growing world population. To tackle this, alternative protein sources need to be evaluated for nutritional value, which requires information on digesta peptide composition in comparison to established protein sources and coupling to...
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Nature Portfolio
2020
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oai:doaj.org-article:8b3824adfd8543ed9a5b564af4ad67392021-12-02T12:42:25ZA combined microphysiological-computational omics approach in dietary protein evaluation10.1038/s41538-020-00082-z2396-8370https://doaj.org/article/8b3824adfd8543ed9a5b564af4ad67392020-12-01T00:00:00Zhttps://doi.org/10.1038/s41538-020-00082-zhttps://doaj.org/toc/2396-8370Abstract Food security is under increased pressure due to the ever-growing world population. To tackle this, alternative protein sources need to be evaluated for nutritional value, which requires information on digesta peptide composition in comparison to established protein sources and coupling to biological parameters. Here, a combined experimental and computational approach is presented, which compared seventeen protein sources with cow’s whey protein concentrate (WPC) as the benchmark. In vitro digestion of proteins was followed by proteomics analysis and statistical model-based clustering. Information on digesta peptide composition resulted in 3 cluster groups, primarily driven by the peptide overlap with the benchmark protein WPC. Functional protein data was then incorporated in the computational model after evaluating the effects of eighteen protein digests on intestinal barrier integrity, viability, brush border enzyme activity, and immune parameters using a bioengineered intestine as microphysiological gut system. This resulted in 6 cluster groups. Biological clustering was driven by viability, brush border enzyme activity, and significant differences in immune parameters. Finally, a combination of proteomic and biological efficacy data resulted in 5 clusters groups, driven by a combination of digesta peptide composition and biological effects. The key finding of our holistic approach is that protein source (animal, plant or alternative derived) is not a driving force behind the delivery of bioactive peptides and their biological efficacy.Paulus G. M. JochemsWillem R. KeustersAntoine H. P. AmericaPascale C. S. RietveldShanna Bastiaan-NetRenata M. C. AriënsMonic M. M. TomassenFraser LewisYang LiKoen G. C. WestphalJohan GarssenHarry J. WichersJeroen van BergenhenegouwenRosalinde MasereeuwNature PortfolioarticleNutrition. Foods and food supplyTX341-641Food processing and manufactureTP368-456ENnpj Science of Food, Vol 4, Iss 1, Pp 1-9 (2020) |
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DOAJ |
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DOAJ |
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EN |
| topic |
Nutrition. Foods and food supply TX341-641 Food processing and manufacture TP368-456 |
| spellingShingle |
Nutrition. Foods and food supply TX341-641 Food processing and manufacture TP368-456 Paulus G. M. Jochems Willem R. Keusters Antoine H. P. America Pascale C. S. Rietveld Shanna Bastiaan-Net Renata M. C. Ariëns Monic M. M. Tomassen Fraser Lewis Yang Li Koen G. C. Westphal Johan Garssen Harry J. Wichers Jeroen van Bergenhenegouwen Rosalinde Masereeuw A combined microphysiological-computational omics approach in dietary protein evaluation |
| description |
Abstract Food security is under increased pressure due to the ever-growing world population. To tackle this, alternative protein sources need to be evaluated for nutritional value, which requires information on digesta peptide composition in comparison to established protein sources and coupling to biological parameters. Here, a combined experimental and computational approach is presented, which compared seventeen protein sources with cow’s whey protein concentrate (WPC) as the benchmark. In vitro digestion of proteins was followed by proteomics analysis and statistical model-based clustering. Information on digesta peptide composition resulted in 3 cluster groups, primarily driven by the peptide overlap with the benchmark protein WPC. Functional protein data was then incorporated in the computational model after evaluating the effects of eighteen protein digests on intestinal barrier integrity, viability, brush border enzyme activity, and immune parameters using a bioengineered intestine as microphysiological gut system. This resulted in 6 cluster groups. Biological clustering was driven by viability, brush border enzyme activity, and significant differences in immune parameters. Finally, a combination of proteomic and biological efficacy data resulted in 5 clusters groups, driven by a combination of digesta peptide composition and biological effects. The key finding of our holistic approach is that protein source (animal, plant or alternative derived) is not a driving force behind the delivery of bioactive peptides and their biological efficacy. |
| format |
article |
| author |
Paulus G. M. Jochems Willem R. Keusters Antoine H. P. America Pascale C. S. Rietveld Shanna Bastiaan-Net Renata M. C. Ariëns Monic M. M. Tomassen Fraser Lewis Yang Li Koen G. C. Westphal Johan Garssen Harry J. Wichers Jeroen van Bergenhenegouwen Rosalinde Masereeuw |
| author_facet |
Paulus G. M. Jochems Willem R. Keusters Antoine H. P. America Pascale C. S. Rietveld Shanna Bastiaan-Net Renata M. C. Ariëns Monic M. M. Tomassen Fraser Lewis Yang Li Koen G. C. Westphal Johan Garssen Harry J. Wichers Jeroen van Bergenhenegouwen Rosalinde Masereeuw |
| author_sort |
Paulus G. M. Jochems |
| title |
A combined microphysiological-computational omics approach in dietary protein evaluation |
| title_short |
A combined microphysiological-computational omics approach in dietary protein evaluation |
| title_full |
A combined microphysiological-computational omics approach in dietary protein evaluation |
| title_fullStr |
A combined microphysiological-computational omics approach in dietary protein evaluation |
| title_full_unstemmed |
A combined microphysiological-computational omics approach in dietary protein evaluation |
| title_sort |
combined microphysiological-computational omics approach in dietary protein evaluation |
| publisher |
Nature Portfolio |
| publishDate |
2020 |
| url |
https://doaj.org/article/8b3824adfd8543ed9a5b564af4ad6739 |
| work_keys_str_mv |
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