Evaluation of 16S rRNA gene sequencing for species and strain-level microbiome analysis

Here, the authors explore the potential of the 16S gene for discriminating bacterial taxa and show that full-length sequencing combined with appropriate clustering of intragenomic sequence variation can provide accurate representation of bacterial species in microbiome datasets.

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Detalles Bibliográficos
Autores principales:	Jethro S. Johnson, Daniel J. Spakowicz, Bo-Young Hong, Lauren M. Petersen, Patrick Demkowicz, Lei Chen, Shana R. Leopold, Blake M. Hanson, Hanako O. Agresta, Mark Gerstein, Erica Sodergren, George M. Weinstock
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/8ba9a0efdb834acd9495876c6b8a6341
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spelling	oai:doaj.org-article:8ba9a0efdb834acd9495876c6b8a63412021-12-02T16:58:28ZEvaluation of 16S rRNA gene sequencing for species and strain-level microbiome analysis10.1038/s41467-019-13036-12041-1723https://doaj.org/article/8ba9a0efdb834acd9495876c6b8a63412019-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-13036-1https://doaj.org/toc/2041-1723Here, the authors explore the potential of the 16S gene for discriminating bacterial taxa and show that full-length sequencing combined with appropriate clustering of intragenomic sequence variation can provide accurate representation of bacterial species in microbiome datasets.Jethro S. JohnsonDaniel J. SpakowiczBo-Young HongLauren M. PetersenPatrick DemkowiczLei ChenShana R. LeopoldBlake M. HansonHanako O. AgrestaMark GersteinErica SodergrenGeorge M. WeinstockNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-11 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Jethro S. Johnson Daniel J. Spakowicz Bo-Young Hong Lauren M. Petersen Patrick Demkowicz Lei Chen Shana R. Leopold Blake M. Hanson Hanako O. Agresta Mark Gerstein Erica Sodergren George M. Weinstock Evaluation of 16S rRNA gene sequencing for species and strain-level microbiome analysis
description	Here, the authors explore the potential of the 16S gene for discriminating bacterial taxa and show that full-length sequencing combined with appropriate clustering of intragenomic sequence variation can provide accurate representation of bacterial species in microbiome datasets.
format	article
author	Jethro S. Johnson Daniel J. Spakowicz Bo-Young Hong Lauren M. Petersen Patrick Demkowicz Lei Chen Shana R. Leopold Blake M. Hanson Hanako O. Agresta Mark Gerstein Erica Sodergren George M. Weinstock
author_facet	Jethro S. Johnson Daniel J. Spakowicz Bo-Young Hong Lauren M. Petersen Patrick Demkowicz Lei Chen Shana R. Leopold Blake M. Hanson Hanako O. Agresta Mark Gerstein Erica Sodergren George M. Weinstock
author_sort	Jethro S. Johnson
title	Evaluation of 16S rRNA gene sequencing for species and strain-level microbiome analysis
title_short	Evaluation of 16S rRNA gene sequencing for species and strain-level microbiome analysis
title_full	Evaluation of 16S rRNA gene sequencing for species and strain-level microbiome analysis
title_fullStr	Evaluation of 16S rRNA gene sequencing for species and strain-level microbiome analysis
title_full_unstemmed	Evaluation of 16S rRNA gene sequencing for species and strain-level microbiome analysis
title_sort	evaluation of 16s rrna gene sequencing for species and strain-level microbiome analysis
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/8ba9a0efdb834acd9495876c6b8a6341
work_keys_str_mv	AT jethrosjohnson evaluationof16srrnagenesequencingforspeciesandstrainlevelmicrobiomeanalysis AT danieljspakowicz evaluationof16srrnagenesequencingforspeciesandstrainlevelmicrobiomeanalysis AT boyounghong evaluationof16srrnagenesequencingforspeciesandstrainlevelmicrobiomeanalysis AT laurenmpetersen evaluationof16srrnagenesequencingforspeciesandstrainlevelmicrobiomeanalysis AT patrickdemkowicz evaluationof16srrnagenesequencingforspeciesandstrainlevelmicrobiomeanalysis AT leichen evaluationof16srrnagenesequencingforspeciesandstrainlevelmicrobiomeanalysis AT shanarleopold evaluationof16srrnagenesequencingforspeciesandstrainlevelmicrobiomeanalysis AT blakemhanson evaluationof16srrnagenesequencingforspeciesandstrainlevelmicrobiomeanalysis AT hanakooagresta evaluationof16srrnagenesequencingforspeciesandstrainlevelmicrobiomeanalysis AT markgerstein evaluationof16srrnagenesequencingforspeciesandstrainlevelmicrobiomeanalysis AT ericasodergren evaluationof16srrnagenesequencingforspeciesandstrainlevelmicrobiomeanalysis AT georgemweinstock evaluationof16srrnagenesequencingforspeciesandstrainlevelmicrobiomeanalysis
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Evaluation of 16S rRNA gene sequencing for species and strain-level microbiome analysis

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