Selective DNAM-1 expression on small peritoneal macrophages contributes to CD4+ T cell costimulation

Abstract Mouse peritoneal macrophages consist of two subsets: large peritoneal macrophages (LPMs) and small peritoneal macrophages (SPMs), defined as CD11bhiF4/80hi and CD11b+F4/80lo cells, respectively. We reveal that SPMs, but not LPMs, have the ability to present antigens to naïve CD4+ T cells. C...

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Autores principales:	Eri Takenaka, Anh Van Vo, Yumi Yamashita-Kanemaru, Akira Shibuya, Kazuko Shibuya
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Peritoneal Macrophages Alum Adjuvant Mediastinal Lymph Nodes Trinitrophenyl (TNP) MHC class-II (MHCII) Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8c34dccbeeeb4050956e420ac25b6467
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spelling	oai:doaj.org-article:8c34dccbeeeb4050956e420ac25b64672021-12-02T15:09:08ZSelective DNAM-1 expression on small peritoneal macrophages contributes to CD4+ T cell costimulation10.1038/s41598-018-33437-42045-2322https://doaj.org/article/8c34dccbeeeb4050956e420ac25b64672018-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-33437-4https://doaj.org/toc/2045-2322Abstract Mouse peritoneal macrophages consist of two subsets: large peritoneal macrophages (LPMs) and small peritoneal macrophages (SPMs), defined as CD11bhiF4/80hi and CD11b+F4/80lo cells, respectively. We reveal that SPMs, but not LPMs, have the ability to present antigens to naïve CD4+ T cells. Coculture of SPMs with naïve ovalbumin (OVA) specific CD4+ T cells (OT-II) in the presence of OVA peptide effectively induced CD4+ T cells priming. SPMs, but not LPMs, strongly express DNAM-1, an activating immunoreceptor. Although antigen uptake and processing were comparable between WT and DNAM-1-deficient SPMs, deficiency of DNAM-1 on SPMs or blockade of DNAM-1 and its ligand interaction impaired CD4+ T cells priming by SPMs. Furthermore, T and B cell responses in mediastinal lymph nodes of mice intraperitoneally immunized with trinitrophenyl (TNP)–OVA protein in Alum adjuvant were enhanced by intraperitoneally transferred wild-type, but not DNAM-1-deficient, SPMs. We propose that SPMs are functionally distinct from LPMs, and DNAM-1 plays a costimulatory role in antigen presentation by SPMs.Eri TakenakaAnh Van VoYumi Yamashita-KanemaruAkira ShibuyaKazuko ShibuyaNature PortfolioarticlePeritoneal MacrophagesAlum AdjuvantMediastinal Lymph NodesTrinitrophenyl (TNP)MHC class-II (MHCII)MedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-8 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Peritoneal Macrophages Alum Adjuvant Mediastinal Lymph Nodes Trinitrophenyl (TNP) MHC class-II (MHCII) Medicine R Science Q
spellingShingle	Peritoneal Macrophages Alum Adjuvant Mediastinal Lymph Nodes Trinitrophenyl (TNP) MHC class-II (MHCII) Medicine R Science Q Eri Takenaka Anh Van Vo Yumi Yamashita-Kanemaru Akira Shibuya Kazuko Shibuya Selective DNAM-1 expression on small peritoneal macrophages contributes to CD4+ T cell costimulation
description	Abstract Mouse peritoneal macrophages consist of two subsets: large peritoneal macrophages (LPMs) and small peritoneal macrophages (SPMs), defined as CD11bhiF4/80hi and CD11b+F4/80lo cells, respectively. We reveal that SPMs, but not LPMs, have the ability to present antigens to naïve CD4+ T cells. Coculture of SPMs with naïve ovalbumin (OVA) specific CD4+ T cells (OT-II) in the presence of OVA peptide effectively induced CD4+ T cells priming. SPMs, but not LPMs, strongly express DNAM-1, an activating immunoreceptor. Although antigen uptake and processing were comparable between WT and DNAM-1-deficient SPMs, deficiency of DNAM-1 on SPMs or blockade of DNAM-1 and its ligand interaction impaired CD4+ T cells priming by SPMs. Furthermore, T and B cell responses in mediastinal lymph nodes of mice intraperitoneally immunized with trinitrophenyl (TNP)–OVA protein in Alum adjuvant were enhanced by intraperitoneally transferred wild-type, but not DNAM-1-deficient, SPMs. We propose that SPMs are functionally distinct from LPMs, and DNAM-1 plays a costimulatory role in antigen presentation by SPMs.
format	article
author	Eri Takenaka Anh Van Vo Yumi Yamashita-Kanemaru Akira Shibuya Kazuko Shibuya
author_facet	Eri Takenaka Anh Van Vo Yumi Yamashita-Kanemaru Akira Shibuya Kazuko Shibuya
author_sort	Eri Takenaka
title	Selective DNAM-1 expression on small peritoneal macrophages contributes to CD4+ T cell costimulation
title_short	Selective DNAM-1 expression on small peritoneal macrophages contributes to CD4+ T cell costimulation
title_full	Selective DNAM-1 expression on small peritoneal macrophages contributes to CD4+ T cell costimulation
title_fullStr	Selective DNAM-1 expression on small peritoneal macrophages contributes to CD4+ T cell costimulation
title_full_unstemmed	Selective DNAM-1 expression on small peritoneal macrophages contributes to CD4+ T cell costimulation
title_sort	selective dnam-1 expression on small peritoneal macrophages contributes to cd4+ t cell costimulation
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/8c34dccbeeeb4050956e420ac25b6467
work_keys_str_mv	AT eritakenaka selectivednam1expressiononsmallperitonealmacrophagescontributestocd4tcellcostimulation AT anhvanvo selectivednam1expressiononsmallperitonealmacrophagescontributestocd4tcellcostimulation AT yumiyamashitakanemaru selectivednam1expressiononsmallperitonealmacrophagescontributestocd4tcellcostimulation AT akirashibuya selectivednam1expressiononsmallperitonealmacrophagescontributestocd4tcellcostimulation AT kazukoshibuya selectivednam1expressiononsmallperitonealmacrophagescontributestocd4tcellcostimulation
_version_	1718387888012394496

Selective DNAM-1 expression on small peritoneal macrophages contributes to CD4+ T cell costimulation

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