DEVELOPMENT OF AN EXTRACTION METHOD OF GOLDEN BERRY (<I>Physalis peruviana</I> L.) POLYPHENOL OXIDASE AND ISOLATION BY AQUEOUS TWO-PHASE SYSTEM

Polyphenol oxidase activity is responsible for the enzymatic browning of fruits and vegetables, and the end products of the catalyzed reaction are detrimental to food quality, in both sensory and nutritional properties. In this study we evaluated the influence of the extraction conditions (pH, conce...

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Autores principales:	Karent E. BRAVO M., Katalina MUÑOZ D., Jénifer CALDERÓN G., Edison J. OSORIO D.
Formato:	article
Lenguaje:	EN
Publicado:	Universidad de Antioquia 2011
Materias:	Golden berry polyphenol oxidase extraction isolation aqueous two-phase systems. Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
Acceso en línea:	https://doaj.org/article/8c7489014f1348908208bd7be2b96654
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spelling	oai:doaj.org-article:8c7489014f1348908208bd7be2b966542021-11-19T04:13:58ZDEVELOPMENT OF AN EXTRACTION METHOD OF GOLDEN BERRY (<I>Physalis peruviana</I> L.) POLYPHENOL OXIDASE AND ISOLATION BY AQUEOUS TWO-PHASE SYSTEM0121-40042145-2660https://doaj.org/article/8c7489014f1348908208bd7be2b966542011-09-01T00:00:00Zhttps://revistas.udea.edu.co/index.php/vitae/article/view/10065https://doaj.org/toc/0121-4004https://doaj.org/toc/2145-2660Polyphenol oxidase activity is responsible for the enzymatic browning of fruits and vegetables, and the end products of the catalyzed reaction are detrimental to food quality, in both sensory and nutritional properties. In this study we evaluated the influence of the extraction conditions (pH, concentration, composition of extraction buffer and protein precipitation agent) on the activity of golden berry polyphenol oxidase. The extraction of proteins with a mixture of Triton® X-100 and polyvinyl pyrrolidone in 0.2 M phosphate buffer at pH 6.0 and precipitation with cold acetone in a ratio supernatant-acetone of 1:1 gave the higher polyphenol oxidase activity. Partial purification of polyphenol oxidase was achieved using an aqueous two-phase system composed by polyethylene glycol and phosphate. Effect of phase composition, molecular weight of polythylene glycol and pH of the system on enzyme partitioning was studied. The optimum system was found at pH 5.5 containing 5% polythylene glycol 8000 and 14% phosphate, with Ka of 4.70, purification factor of 4.65 and a 74.29% yield of enzyme activity in the top phase. These results show a significant effect of extraction conditions on polyphenol oxidase activity and provide a quick, simple and efficient method for polyphenol oxidase isolation.Karent E. BRAVO M.Katalina MUÑOZ D.Jénifer CALDERÓN G.Edison J. OSORIO D.Universidad de AntioquiaarticleGolden berrypolyphenol oxidaseextractionisolationaqueous two-phase systems.Food processing and manufactureTP368-456Pharmaceutical industryHD9665-9675ENVitae, Vol 18, Iss 2 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Golden berry polyphenol oxidase extraction isolation aqueous two-phase systems. Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
spellingShingle	Golden berry polyphenol oxidase extraction isolation aqueous two-phase systems. Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675 Karent E. BRAVO M. Katalina MUÑOZ D. Jénifer CALDERÓN G. Edison J. OSORIO D. DEVELOPMENT OF AN EXTRACTION METHOD OF GOLDEN BERRY (<I>Physalis peruviana</I> L.) POLYPHENOL OXIDASE AND ISOLATION BY AQUEOUS TWO-PHASE SYSTEM
description	Polyphenol oxidase activity is responsible for the enzymatic browning of fruits and vegetables, and the end products of the catalyzed reaction are detrimental to food quality, in both sensory and nutritional properties. In this study we evaluated the influence of the extraction conditions (pH, concentration, composition of extraction buffer and protein precipitation agent) on the activity of golden berry polyphenol oxidase. The extraction of proteins with a mixture of Triton® X-100 and polyvinyl pyrrolidone in 0.2 M phosphate buffer at pH 6.0 and precipitation with cold acetone in a ratio supernatant-acetone of 1:1 gave the higher polyphenol oxidase activity. Partial purification of polyphenol oxidase was achieved using an aqueous two-phase system composed by polyethylene glycol and phosphate. Effect of phase composition, molecular weight of polythylene glycol and pH of the system on enzyme partitioning was studied. The optimum system was found at pH 5.5 containing 5% polythylene glycol 8000 and 14% phosphate, with Ka of 4.70, purification factor of 4.65 and a 74.29% yield of enzyme activity in the top phase. These results show a significant effect of extraction conditions on polyphenol oxidase activity and provide a quick, simple and efficient method for polyphenol oxidase isolation.
format	article
author	Karent E. BRAVO M. Katalina MUÑOZ D. Jénifer CALDERÓN G. Edison J. OSORIO D.
author_facet	Karent E. BRAVO M. Katalina MUÑOZ D. Jénifer CALDERÓN G. Edison J. OSORIO D.
author_sort	Karent E. BRAVO M.
title	DEVELOPMENT OF AN EXTRACTION METHOD OF GOLDEN BERRY (<I>Physalis peruviana</I> L.) POLYPHENOL OXIDASE AND ISOLATION BY AQUEOUS TWO-PHASE SYSTEM
title_short	DEVELOPMENT OF AN EXTRACTION METHOD OF GOLDEN BERRY (<I>Physalis peruviana</I> L.) POLYPHENOL OXIDASE AND ISOLATION BY AQUEOUS TWO-PHASE SYSTEM
title_full	DEVELOPMENT OF AN EXTRACTION METHOD OF GOLDEN BERRY (<I>Physalis peruviana</I> L.) POLYPHENOL OXIDASE AND ISOLATION BY AQUEOUS TWO-PHASE SYSTEM
title_fullStr	DEVELOPMENT OF AN EXTRACTION METHOD OF GOLDEN BERRY (<I>Physalis peruviana</I> L.) POLYPHENOL OXIDASE AND ISOLATION BY AQUEOUS TWO-PHASE SYSTEM
title_full_unstemmed	DEVELOPMENT OF AN EXTRACTION METHOD OF GOLDEN BERRY (<I>Physalis peruviana</I> L.) POLYPHENOL OXIDASE AND ISOLATION BY AQUEOUS TWO-PHASE SYSTEM
title_sort	development of an extraction method of golden berry (<i>physalis peruviana</i> l.) polyphenol oxidase and isolation by aqueous two-phase system
publisher	Universidad de Antioquia
publishDate	2011
url	https://doaj.org/article/8c7489014f1348908208bd7be2b96654
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DEVELOPMENT OF AN EXTRACTION METHOD OF GOLDEN BERRY (<I>Physalis peruviana</I> L.) POLYPHENOL OXIDASE AND ISOLATION BY AQUEOUS TWO-PHASE SYSTEM

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