Multiplexed pancreatic genome engineering and cancer induction by transfection-based CRISPR/Cas9 delivery in mice
CRISPR/Cas9 technology has been used for genome engineering in vivo. Here, the authors use a transfection technique to deliver multiple guide RNAs to the pancreas of adult mice, allowing genetic screening and chromosome engineering in pancreatic cancer.
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Nature Portfolio
2016
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oai:doaj.org-article:8d54419326794a6ba55ff4332e373a042021-12-02T14:39:51ZMultiplexed pancreatic genome engineering and cancer induction by transfection-based CRISPR/Cas9 delivery in mice10.1038/ncomms107702041-1723https://doaj.org/article/8d54419326794a6ba55ff4332e373a042016-02-01T00:00:00Zhttps://doi.org/10.1038/ncomms10770https://doaj.org/toc/2041-1723CRISPR/Cas9 technology has been used for genome engineering in vivo. Here, the authors use a transfection technique to deliver multiple guide RNAs to the pancreas of adult mice, allowing genetic screening and chromosome engineering in pancreatic cancer.Roman MareschSebastian MuellerChristian VeltkampRupert ÖllingerMathias FriedrichIrina HeidKatja SteigerJulia WeberThomas EngleitnerMaxim BarenboimSabine KleinSandra LouzadaRuby BanerjeeAlexander StrongTeresa StauberNina GrossUlf GeumannSebastian LangeMarc RingelhanIgnacio VarelaKristian UngerFengtang YangRoland M. SchmidGeorge S. VassiliouRickmer BrarenGünter SchneiderMathias HeikenwalderAllan BradleyDieter SaurRoland RadNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-13 (2016) |
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DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Science Q |
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Science Q Roman Maresch Sebastian Mueller Christian Veltkamp Rupert Öllinger Mathias Friedrich Irina Heid Katja Steiger Julia Weber Thomas Engleitner Maxim Barenboim Sabine Klein Sandra Louzada Ruby Banerjee Alexander Strong Teresa Stauber Nina Gross Ulf Geumann Sebastian Lange Marc Ringelhan Ignacio Varela Kristian Unger Fengtang Yang Roland M. Schmid George S. Vassiliou Rickmer Braren Günter Schneider Mathias Heikenwalder Allan Bradley Dieter Saur Roland Rad Multiplexed pancreatic genome engineering and cancer induction by transfection-based CRISPR/Cas9 delivery in mice |
| description |
CRISPR/Cas9 technology has been used for genome engineering in vivo. Here, the authors use a transfection technique to deliver multiple guide RNAs to the pancreas of adult mice, allowing genetic screening and chromosome engineering in pancreatic cancer. |
| format |
article |
| author |
Roman Maresch Sebastian Mueller Christian Veltkamp Rupert Öllinger Mathias Friedrich Irina Heid Katja Steiger Julia Weber Thomas Engleitner Maxim Barenboim Sabine Klein Sandra Louzada Ruby Banerjee Alexander Strong Teresa Stauber Nina Gross Ulf Geumann Sebastian Lange Marc Ringelhan Ignacio Varela Kristian Unger Fengtang Yang Roland M. Schmid George S. Vassiliou Rickmer Braren Günter Schneider Mathias Heikenwalder Allan Bradley Dieter Saur Roland Rad |
| author_facet |
Roman Maresch Sebastian Mueller Christian Veltkamp Rupert Öllinger Mathias Friedrich Irina Heid Katja Steiger Julia Weber Thomas Engleitner Maxim Barenboim Sabine Klein Sandra Louzada Ruby Banerjee Alexander Strong Teresa Stauber Nina Gross Ulf Geumann Sebastian Lange Marc Ringelhan Ignacio Varela Kristian Unger Fengtang Yang Roland M. Schmid George S. Vassiliou Rickmer Braren Günter Schneider Mathias Heikenwalder Allan Bradley Dieter Saur Roland Rad |
| author_sort |
Roman Maresch |
| title |
Multiplexed pancreatic genome engineering and cancer induction by transfection-based CRISPR/Cas9 delivery in mice |
| title_short |
Multiplexed pancreatic genome engineering and cancer induction by transfection-based CRISPR/Cas9 delivery in mice |
| title_full |
Multiplexed pancreatic genome engineering and cancer induction by transfection-based CRISPR/Cas9 delivery in mice |
| title_fullStr |
Multiplexed pancreatic genome engineering and cancer induction by transfection-based CRISPR/Cas9 delivery in mice |
| title_full_unstemmed |
Multiplexed pancreatic genome engineering and cancer induction by transfection-based CRISPR/Cas9 delivery in mice |
| title_sort |
multiplexed pancreatic genome engineering and cancer induction by transfection-based crispr/cas9 delivery in mice |
| publisher |
Nature Portfolio |
| publishDate |
2016 |
| url |
https://doaj.org/article/8d54419326794a6ba55ff4332e373a04 |
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