Anatomical structure, flavonoid content, and antioxidant activity of Rhodomyrtus tomentosa leaves and fruits on different age and maturity level

Abstract. Kuntorini EM, Nugroho LH, Maryani, Nuringtyas TR. 2019. Anatomical structure, flavonoid content, and antioxidant activity of Rhodomyrtus tomentosa leaves and fruits on different age and maturity level. Biodiversitas 20: 3619-3625. Karamunting (Rhodomyrtus tomentosa (Aiton.) Hassk.) is a na...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	evi kuntorini, LAURENTIUS HARTANTO NUGROHO, MARYANI, TRI RINI NURINGTYAS
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	anatomical structure, antioxidant activity, histochemical content, rhodomyrtus tomentosa Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/8ee3abc63e3045c89ea094b5b8d5051a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8ee3abc63e3045c89ea094b5b8d5051a
record_format	dspace
spelling	oai:doaj.org-article:8ee3abc63e3045c89ea094b5b8d5051a2021-11-21T21:59:05ZAnatomical structure, flavonoid content, and antioxidant activity of Rhodomyrtus tomentosa leaves and fruits on different age and maturity level1412-033X2085-472210.13057/biodiv/d201221https://doaj.org/article/8ee3abc63e3045c89ea094b5b8d5051a2019-11-01T00:00:00Zhttps://smujo.id/biodiv/article/view/4596https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Kuntorini EM, Nugroho LH, Maryani, Nuringtyas TR. 2019. Anatomical structure, flavonoid content, and antioxidant activity of Rhodomyrtus tomentosa leaves and fruits on different age and maturity level. Biodiversitas 20: 3619-3625. Karamunting (Rhodomyrtus tomentosa (Aiton.) Hassk.) is a native plant to southeast Asian countries, and is a well-known medicinal plant used to treat colic diarrhea, wounds, heartburn, abscesses, gynecopathy, and as a pain killer. However, the use of R. tomentosa has not been optimized. This research aimed to observe the anatomical structure, the location, and distribution of flavonoid and to find out the antioxidant activity based on the leaves age and the fruit maturity. Anatomical slides preparation of leaves and fruits were made using the paraffin embedding method with safranin staining. The distribution of flavonoid was analyzed by histochemical test and antioxidant activity was done with DPPH (1,1-diphenyl-2-picrylhydrazyl) method. Leaf anatomical structure shows that the leaf bifacial (dorsiventral) consisted of upper epidermis, mesophyll (palisade and sponge), collateral vascular bundle, parenchyma midrib, abaxial epidermis, globular oil cavity, and non-glandular trichome. Transverse section of green fruit consists of exocarp (thin outer layer), mesocarp (thick inner layer, soft and runny) and endocarp (thin false septa) layers. The histochemical test showed that flavonoid was observed in the leaf epidermis, mesophyll, vascular bundles, secretory cavity, parenchyma and in all fruit types exocarp, mesocarp, endocarp. Antioxidant activity showed that the extract of the young leaves (IC50 = 14.67 ppm) was stronger than the old leaves (IC50 = 19.86 ppm). The antioxidant activity of the purple fruits extract (IC50 = 12.98 ppm) was stronger than the red fruits (IC50 = 28.63 ppm) and the green fruits (IC50 = 48.36 ppm) but it was weaker than quercetin (IC50 = 1.29 ppm). The purple fruit had the highest antioxidant activity compared to other extracts. This information will be useful for developing karamunting as a potential resource of natural antioxidants for functional foods and health products.evi kuntoriniLAURENTIUS HARTANTO NUGROHOMARYANITRI RINI NURINGTYASMBI & UNS Soloarticleanatomical structure, antioxidant activity, histochemical content, rhodomyrtus tomentosaBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 12 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	anatomical structure, antioxidant activity, histochemical content, rhodomyrtus tomentosa Biology (General) QH301-705.5
spellingShingle	anatomical structure, antioxidant activity, histochemical content, rhodomyrtus tomentosa Biology (General) QH301-705.5 evi kuntorini LAURENTIUS HARTANTO NUGROHO MARYANI TRI RINI NURINGTYAS Anatomical structure, flavonoid content, and antioxidant activity of Rhodomyrtus tomentosa leaves and fruits on different age and maturity level
description	Abstract. Kuntorini EM, Nugroho LH, Maryani, Nuringtyas TR. 2019. Anatomical structure, flavonoid content, and antioxidant activity of Rhodomyrtus tomentosa leaves and fruits on different age and maturity level. Biodiversitas 20: 3619-3625. Karamunting (Rhodomyrtus tomentosa (Aiton.) Hassk.) is a native plant to southeast Asian countries, and is a well-known medicinal plant used to treat colic diarrhea, wounds, heartburn, abscesses, gynecopathy, and as a pain killer. However, the use of R. tomentosa has not been optimized. This research aimed to observe the anatomical structure, the location, and distribution of flavonoid and to find out the antioxidant activity based on the leaves age and the fruit maturity. Anatomical slides preparation of leaves and fruits were made using the paraffin embedding method with safranin staining. The distribution of flavonoid was analyzed by histochemical test and antioxidant activity was done with DPPH (1,1-diphenyl-2-picrylhydrazyl) method. Leaf anatomical structure shows that the leaf bifacial (dorsiventral) consisted of upper epidermis, mesophyll (palisade and sponge), collateral vascular bundle, parenchyma midrib, abaxial epidermis, globular oil cavity, and non-glandular trichome. Transverse section of green fruit consists of exocarp (thin outer layer), mesocarp (thick inner layer, soft and runny) and endocarp (thin false septa) layers. The histochemical test showed that flavonoid was observed in the leaf epidermis, mesophyll, vascular bundles, secretory cavity, parenchyma and in all fruit types exocarp, mesocarp, endocarp. Antioxidant activity showed that the extract of the young leaves (IC50 = 14.67 ppm) was stronger than the old leaves (IC50 = 19.86 ppm). The antioxidant activity of the purple fruits extract (IC50 = 12.98 ppm) was stronger than the red fruits (IC50 = 28.63 ppm) and the green fruits (IC50 = 48.36 ppm) but it was weaker than quercetin (IC50 = 1.29 ppm). The purple fruit had the highest antioxidant activity compared to other extracts. This information will be useful for developing karamunting as a potential resource of natural antioxidants for functional foods and health products.
format	article
author	evi kuntorini LAURENTIUS HARTANTO NUGROHO MARYANI TRI RINI NURINGTYAS
author_facet	evi kuntorini LAURENTIUS HARTANTO NUGROHO MARYANI TRI RINI NURINGTYAS
author_sort	evi kuntorini
title	Anatomical structure, flavonoid content, and antioxidant activity of Rhodomyrtus tomentosa leaves and fruits on different age and maturity level
title_short	Anatomical structure, flavonoid content, and antioxidant activity of Rhodomyrtus tomentosa leaves and fruits on different age and maturity level
title_full	Anatomical structure, flavonoid content, and antioxidant activity of Rhodomyrtus tomentosa leaves and fruits on different age and maturity level
title_fullStr	Anatomical structure, flavonoid content, and antioxidant activity of Rhodomyrtus tomentosa leaves and fruits on different age and maturity level
title_full_unstemmed	Anatomical structure, flavonoid content, and antioxidant activity of Rhodomyrtus tomentosa leaves and fruits on different age and maturity level
title_sort	anatomical structure, flavonoid content, and antioxidant activity of rhodomyrtus tomentosa leaves and fruits on different age and maturity level
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/8ee3abc63e3045c89ea094b5b8d5051a
work_keys_str_mv	AT evikuntorini anatomicalstructureflavonoidcontentandantioxidantactivityofrhodomyrtustomentosaleavesandfruitsondifferentageandmaturitylevel AT laurentiushartantonugroho anatomicalstructureflavonoidcontentandantioxidantactivityofrhodomyrtustomentosaleavesandfruitsondifferentageandmaturitylevel AT maryani anatomicalstructureflavonoidcontentandantioxidantactivityofrhodomyrtustomentosaleavesandfruitsondifferentageandmaturitylevel AT tririninuringtyas anatomicalstructureflavonoidcontentandantioxidantactivityofrhodomyrtustomentosaleavesandfruitsondifferentageandmaturitylevel
_version_	1718418594880028672

Anatomical structure, flavonoid content, and antioxidant activity of Rhodomyrtus tomentosa leaves and fruits on different age and maturity level

Ejemplares similares