De novo identification of essential protein domains from CRISPR-Cas9 tiling-sgRNA knockout screens

De novo identification of essential protein domains from CRISPR-Cas9 tiling-sgRNA knockout screens

Tiling-sgRNA designs allow the in situ evaluation of protein domain functions. Here the authors present ProTiler - a computational method to predict CRISPR knockout hyper-sensitive regions, revealing previously unannotated domains.

Saved in:
Bibliographic Details
Main Authors: Wei He, Liang Zhang, Oscar D. Villarreal, Rongjie Fu, Ella Bedford, Jingzhuang Dou, Anish Y. Patel, Mark T. Bedford, Xiaobing Shi, Taiping Chen, Blaine Bartholomew, Han Xu
Format: article
Language:EN
Published: Nature Portfolio 2019
Subjects:
Science
Q
Online Access:https://doaj.org/article/8f9bec4646d34e8fa3bf3716402d6050
Tags: Add Tag
No Tags, Be the first to tag this record!

Internet

https://doaj.org/article/8f9bec4646d34e8fa3bf3716402d6050

Similar Items