Akt mediates metastasis-associated gene 1 (MTA1) regulating the expression of E-cadherin and promoting the invasiveness of prostate cancer cells.
Human metastasis-associated gene 1 (MTA1) is highly associated with the metastasis of prostate cancer; however, the molecular functions of MTA1 that facilitate metastasis remain unclear. In this study, we demonstrate that the silencing of MTA1 by siRNA treatment results in the upregulation of E-cadh...
Guardado en:
| Autores principales: | , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2012
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/9013904d69cd4932ba77490f17698838 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:9013904d69cd4932ba77490f17698838 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:9013904d69cd4932ba77490f176988382021-11-18T08:06:23ZAkt mediates metastasis-associated gene 1 (MTA1) regulating the expression of E-cadherin and promoting the invasiveness of prostate cancer cells.1932-620310.1371/journal.pone.0046888https://doaj.org/article/9013904d69cd4932ba77490f176988382012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23227138/?tool=EBIhttps://doaj.org/toc/1932-6203Human metastasis-associated gene 1 (MTA1) is highly associated with the metastasis of prostate cancer; however, the molecular functions of MTA1 that facilitate metastasis remain unclear. In this study, we demonstrate that the silencing of MTA1 by siRNA treatment results in the upregulation of E-cadherin expression by the phosphorylation of AKT (p-AKT) and decreases the invasiveness of prostate cancer cells. We show that MTA1 is expressed in over 90% of prostate cancer tissues, especially metastatic prostate cancer tissue, comparing to non-expression in normal prostate tissue. RT-PCR analysis and Western blot assay showed that MTA1 expression is significantly higher in highly metastatic prostate cancer PC-3M-1E8 cells (1E8) than in poorly metastatic prostate cancer PC-3M-2B4 cells (2B4). Silencing MTA1 expression by siRNA treatment in 1E8 cells increased the cellular malignant characters, including the cellular adhesive ability, decreased the cellular invasive ability and changed the polarity of cellular cytoskeleton. 1E8 cells over-expressing MTA1 had a reduced expression of E-cadherin, while 1E8 cells treated with MTA1 siRNA had a higher expression of E-cadherin. The expression of phosphorylated AKT (p-AKT) or the inhibition of p-AKT by wortmannin treatment (100 nM) significantly altered the function of MTA1 in the regulation of E-cadherin expression. Alterations in E-cadherin expression changed the role of p-AKT in cellular malignant characters. All of these results demonstrate that MTA1 plays an important role in controlling the malignant transformation of prostate cancer cells through the p-AKT/E-cadherin pathway. This study also provides a new mechanistic role for MTA1 in the regulation of prostate cancer metastasis.Hongyan WangLiangsheng FanJuncheng WeiYanjie WengLi ZhouYing ShiWenjuan ZhouDing MaChangyu WangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 12, p e46888 (2012) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Hongyan Wang Liangsheng Fan Juncheng Wei Yanjie Weng Li Zhou Ying Shi Wenjuan Zhou Ding Ma Changyu Wang Akt mediates metastasis-associated gene 1 (MTA1) regulating the expression of E-cadherin and promoting the invasiveness of prostate cancer cells. |
| description |
Human metastasis-associated gene 1 (MTA1) is highly associated with the metastasis of prostate cancer; however, the molecular functions of MTA1 that facilitate metastasis remain unclear. In this study, we demonstrate that the silencing of MTA1 by siRNA treatment results in the upregulation of E-cadherin expression by the phosphorylation of AKT (p-AKT) and decreases the invasiveness of prostate cancer cells. We show that MTA1 is expressed in over 90% of prostate cancer tissues, especially metastatic prostate cancer tissue, comparing to non-expression in normal prostate tissue. RT-PCR analysis and Western blot assay showed that MTA1 expression is significantly higher in highly metastatic prostate cancer PC-3M-1E8 cells (1E8) than in poorly metastatic prostate cancer PC-3M-2B4 cells (2B4). Silencing MTA1 expression by siRNA treatment in 1E8 cells increased the cellular malignant characters, including the cellular adhesive ability, decreased the cellular invasive ability and changed the polarity of cellular cytoskeleton. 1E8 cells over-expressing MTA1 had a reduced expression of E-cadherin, while 1E8 cells treated with MTA1 siRNA had a higher expression of E-cadherin. The expression of phosphorylated AKT (p-AKT) or the inhibition of p-AKT by wortmannin treatment (100 nM) significantly altered the function of MTA1 in the regulation of E-cadherin expression. Alterations in E-cadherin expression changed the role of p-AKT in cellular malignant characters. All of these results demonstrate that MTA1 plays an important role in controlling the malignant transformation of prostate cancer cells through the p-AKT/E-cadherin pathway. This study also provides a new mechanistic role for MTA1 in the regulation of prostate cancer metastasis. |
| format |
article |
| author |
Hongyan Wang Liangsheng Fan Juncheng Wei Yanjie Weng Li Zhou Ying Shi Wenjuan Zhou Ding Ma Changyu Wang |
| author_facet |
Hongyan Wang Liangsheng Fan Juncheng Wei Yanjie Weng Li Zhou Ying Shi Wenjuan Zhou Ding Ma Changyu Wang |
| author_sort |
Hongyan Wang |
| title |
Akt mediates metastasis-associated gene 1 (MTA1) regulating the expression of E-cadherin and promoting the invasiveness of prostate cancer cells. |
| title_short |
Akt mediates metastasis-associated gene 1 (MTA1) regulating the expression of E-cadherin and promoting the invasiveness of prostate cancer cells. |
| title_full |
Akt mediates metastasis-associated gene 1 (MTA1) regulating the expression of E-cadherin and promoting the invasiveness of prostate cancer cells. |
| title_fullStr |
Akt mediates metastasis-associated gene 1 (MTA1) regulating the expression of E-cadherin and promoting the invasiveness of prostate cancer cells. |
| title_full_unstemmed |
Akt mediates metastasis-associated gene 1 (MTA1) regulating the expression of E-cadherin and promoting the invasiveness of prostate cancer cells. |
| title_sort |
akt mediates metastasis-associated gene 1 (mta1) regulating the expression of e-cadherin and promoting the invasiveness of prostate cancer cells. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2012 |
| url |
https://doaj.org/article/9013904d69cd4932ba77490f17698838 |
| work_keys_str_mv |
AT hongyanwang aktmediatesmetastasisassociatedgene1mta1regulatingtheexpressionofecadherinandpromotingtheinvasivenessofprostatecancercells AT liangshengfan aktmediatesmetastasisassociatedgene1mta1regulatingtheexpressionofecadherinandpromotingtheinvasivenessofprostatecancercells AT junchengwei aktmediatesmetastasisassociatedgene1mta1regulatingtheexpressionofecadherinandpromotingtheinvasivenessofprostatecancercells AT yanjieweng aktmediatesmetastasisassociatedgene1mta1regulatingtheexpressionofecadherinandpromotingtheinvasivenessofprostatecancercells AT lizhou aktmediatesmetastasisassociatedgene1mta1regulatingtheexpressionofecadherinandpromotingtheinvasivenessofprostatecancercells AT yingshi aktmediatesmetastasisassociatedgene1mta1regulatingtheexpressionofecadherinandpromotingtheinvasivenessofprostatecancercells AT wenjuanzhou aktmediatesmetastasisassociatedgene1mta1regulatingtheexpressionofecadherinandpromotingtheinvasivenessofprostatecancercells AT dingma aktmediatesmetastasisassociatedgene1mta1regulatingtheexpressionofecadherinandpromotingtheinvasivenessofprostatecancercells AT changyuwang aktmediatesmetastasisassociatedgene1mta1regulatingtheexpressionofecadherinandpromotingtheinvasivenessofprostatecancercells |
| _version_ |
1718422243319480320 |