Accessing unexplored regions of sequence space in directed enzyme evolution via insertion/deletion mutagenesis

Insertions/Deletions (InDels) remain an untapped source of protein diversity in laboratory evolution. The method TRIAD generates libraries of random variants with short in-frame InDels using transposons, allowing a comparison of their evolutionary potential with widely-used point mutant libraries.

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Autores principales: Stephane Emond, Maya Petek, Emily J. Kay, Brennen Heames, Sean R. A. Devenish, Nobuhiko Tokuriki, Florian Hollfelder
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2020
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Acceso en línea:https://doaj.org/article/90b00a5d4c434606bdb4fa1cf03f971e
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