Accessing unexplored regions of sequence space in directed enzyme evolution via insertion/deletion mutagenesis

Insertions/Deletions (InDels) remain an untapped source of protein diversity in laboratory evolution. The method TRIAD generates libraries of random variants with short in-frame InDels using transposons, allowing a comparison of their evolutionary potential with widely-used point mutant libraries.

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Autores principales:	Stephane Emond, Maya Petek, Emily J. Kay, Brennen Heames, Sean R. A. Devenish, Nobuhiko Tokuriki, Florian Hollfelder
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/90b00a5d4c434606bdb4fa1cf03f971e
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spelling	oai:doaj.org-article:90b00a5d4c434606bdb4fa1cf03f971e2021-12-02T16:24:52ZAccessing unexplored regions of sequence space in directed enzyme evolution via insertion/deletion mutagenesis10.1038/s41467-020-17061-32041-1723https://doaj.org/article/90b00a5d4c434606bdb4fa1cf03f971e2020-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-17061-3https://doaj.org/toc/2041-1723Insertions/Deletions (InDels) remain an untapped source of protein diversity in laboratory evolution. The method TRIAD generates libraries of random variants with short in-frame InDels using transposons, allowing a comparison of their evolutionary potential with widely-used point mutant libraries.Stephane EmondMaya PetekEmily J. KayBrennen HeamesSean R. A. DevenishNobuhiko TokurikiFlorian HollfelderNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-14 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Stephane Emond Maya Petek Emily J. Kay Brennen Heames Sean R. A. Devenish Nobuhiko Tokuriki Florian Hollfelder Accessing unexplored regions of sequence space in directed enzyme evolution via insertion/deletion mutagenesis
description	Insertions/Deletions (InDels) remain an untapped source of protein diversity in laboratory evolution. The method TRIAD generates libraries of random variants with short in-frame InDels using transposons, allowing a comparison of their evolutionary potential with widely-used point mutant libraries.
format	article
author	Stephane Emond Maya Petek Emily J. Kay Brennen Heames Sean R. A. Devenish Nobuhiko Tokuriki Florian Hollfelder
author_facet	Stephane Emond Maya Petek Emily J. Kay Brennen Heames Sean R. A. Devenish Nobuhiko Tokuriki Florian Hollfelder
author_sort	Stephane Emond
title	Accessing unexplored regions of sequence space in directed enzyme evolution via insertion/deletion mutagenesis
title_short	Accessing unexplored regions of sequence space in directed enzyme evolution via insertion/deletion mutagenesis
title_full	Accessing unexplored regions of sequence space in directed enzyme evolution via insertion/deletion mutagenesis
title_fullStr	Accessing unexplored regions of sequence space in directed enzyme evolution via insertion/deletion mutagenesis
title_full_unstemmed	Accessing unexplored regions of sequence space in directed enzyme evolution via insertion/deletion mutagenesis
title_sort	accessing unexplored regions of sequence space in directed enzyme evolution via insertion/deletion mutagenesis
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/90b00a5d4c434606bdb4fa1cf03f971e
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Accessing unexplored regions of sequence space in directed enzyme evolution via insertion/deletion mutagenesis

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